Rad21 Antibody - BSA Free

Rad21 Antibody [NB100-203] - Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Rad21 antibody NB100-203 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Rad21 Antibody [NB100-203] - Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Rad21 antibody NB100-203 (Lot NB100-203-8) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot NB100-203-6). For blotting immunoprecipitated Rad21, rabbit anti-Rad21 recombinant monoclonal antibody NB100-203 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.Previous

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Rad21 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Rad21 Antibody - BSA Free (NB100-203) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 10 ug/mg of lysate
  • Western Blot 1:500-1:5000
• Control:

  Rad21 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad21 Antibody - BSA Free

• double-strand-break repair protein rad21 homolog
• hHR21FLJ25655
• HR21
• KIAA0078RAD21 (S. pombe) homolog
• MCD1
• Nuclear matrix protein 1
• NXP-1
• NXP1HRAD21
• protein involved in DNA double-strand break repair
• RAD21 homolog (S. pombe)
• SCC1 homolog
• SCC1FLJ40596

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD21