Rad21 Antibody (CM110-2C10) - Azide and BSA Free

Western Blot: Rad21 Antibody (CM110-2C10) - Azide and BSA Free [NBP2-80922] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates. Image from the standard format of this antibody.

Simple Western: Rad21 Antibody (CM110-2C10) - Azide and BSA Free [NBP2-80922] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western
• Clone: CM110-2C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Rad21 Antibody (CM110-2C10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Rad21 Antibody (CM110-2C10) - Azide and BSA Free (NB100-386) is a monoclonal antibody validated for use in WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
• Localization: Nuclear and cytoplasm
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD21
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:50
  • Western Blot 1:500 (ECL)
• Application Notes: This Rad21 (CM110-2C10) antibody can be used in Western blot, where two bands are seen. The full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Rad21 Antibody (CM110-2C10) - Azide and BSA Free

• double-strand-break repair protein rad21 homolog
• hHR21FLJ25655
• HR21
• KIAA0078RAD21 (S. pombe) homolog
• MCD1
• Nuclear matrix protein 1
• NXP-1
• NXP1HRAD21
• protein involved in DNA double-strand break repair
• RAD21 homolog (S. pombe)
• SCC1 homolog
• SCC1FLJ40596

Background

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD21