Rac1 [p Ser71] Antibody - BSA Free

Western Blot: Rac1 [p Ser71] Antibody [NBP3-38637] - Western blot analysis of lysates from A-431 cells, using Rac1 Rabbit pAb at 1:1000 dilution. A-431 cells were treated by EGF (25 ug/mL) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Rac1 [p Ser71] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Rac1 [p Ser71] Antibody - BSA Free (NBP3-38637) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S71 of human Rac1 (NP_008839.2).
  
  Sequence:
  PLSYP
• Modification: p Ser71
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Rac1 [p Ser71] Antibody - BSA Free

• Cell migration-inducing gene 5 protein
• MGC111543
• p21-Rac1
• p21-Rac1migration-inducing gene 5
• Rac1
• Ras-like protein TC25
• ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1)
• ras-related C3 botulinum toxin substrate 1
• rho family, small GTP binding protein Rac1
• TC25
• TC-25

Background

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAC1