Recombinant Human Rab5b His Protein

Recombinant Human Rab5b Protein [NBP1-50930]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Rab5b His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-215 of Human Rab5b
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTSRSTA RPNGQPQASK ICQFKLVLLG ESAVGKSSLV LRFVKGQFHE YQESTIGAAF LTQSVCLDDT TVKFEIWDTA GQERYHSLAP MYYRGAQAAI VVYDITNQET FARAKTWVKE LQRQASPSIV IALAGNKADL ANKRMVEYEE AQAYADDNSL LFMETSAKTA MNVNDLFLAI AKKLPKSEPQ NLGGAAGRSR GVDLHEQSQQ NKSQCCSN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RAB5B
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 26.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Rab5b His Protein

• RAB5B, member RAS oncogene family
• ras-related protein Rab-5B

Background

Ras-related protein Rab-5B, also known as RAB5B, is a member of the Rab family of small (monomeric) G proteins. RAB5B is involved in endocytosis and recycling of cell surface molecules. It interacts with RIN2 and RIN3, which regulate its function, possibly by acting as GEFs. Knockdown of RAB5B abolished group I metabotropic glutamate receptor (mGluR)-mediated neuroprotection. Recombinant human RAB5B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RAB5B