Recombinant Human Rab5a Protein Summary
| Description |
An un-tagged recombinant protein corresponding to the amino acids 1-215 of Human Rab5a Source: E.coli Amino Acid Sequence: MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD LTEPTQPTRN QCCSN |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
RAB5A |
| Purity |
>95%, by SDS-PAGE |
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20mM MES buffer (pH6.0), 10% glycerol, 0.1mM PMSF |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human Rab5a Protein
Background
Rab5a is a member of RAS oncogene family. The small GTPase, Rab5a is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex. Recombinant human Rab5a was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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