Rab5a Antibody

Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NBP1-20255] - Mouse bone marrow derived DCs stained with anti-Rab5a antibody. ICC/IF image submitted by a verified customer review.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Rab5a Antibody Summary

• Description: Novus Biologicals Sheep Rab5a Antibody (NBP1-20255) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Rab5a Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from the internal region of mouse Rab5a conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in rat.
• Marker: Early Endosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: RAB5A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Western Blot 1:300 - 1:2000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 24554775).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Rab5a Antibody

• RAB5A, member RAS oncogene family
• RAB5RAS-associated protein RAB5A
• ras-related protein Rab-5A

Background

FUNCTION: Required for the fusion of plasma membranes and early endosomes.; ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab5a Antibody (NBP1-20255)(2)

Publications using NBP1-20255

• Subhankar Dolai, Tao Liang, Abrahim I Orabi, Douglas Holmyard, Li Xie, Dafna Greitzer-Antes, Youhou Kang, Huanli Xie, Tanveer A Javed, Patrick P Lam, Deborah C Rubin, Peter Thorn, Herbert Y Gaisano Pancreatitis-Induced Depletion of Syntaxin 2 Promotes Autophagy and Increases Basolateral Exocytosis. Gastroenterology 2018-06-13 [PMID: 29360461]
• Cao W, Ramakrishnan R, Tuyrin VA et al. Oxidized Lipids Block Antigen Cross-Presentation by Dendritic Cells in Cancer. J. Immunol. 2014-02-20 [PMID: 24554775] (ICC/IF, Mouse)

Review for Rab5a Antibody (NBP1-20255) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 04/02/2015

Summary

• Application: Immunofluorescence
• Sample Tested: mouse bone marrow derived DCs
• Species: Mouse
• Lot: NBP1-20255

Bioinformatics

• Gene Symbol: RAB5A
• Entrez:
  • 271457(Mouse)
  • 64633(Rat)
• Uniprot:
  • Q9CQD1(Mouse)