Rab5a Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NBP1-20255] - Mouse bone marrow derived DCs stained with anti-Rab5a antibody. Image from verified customer review.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Rab5a Antibody Summary

Immunogen
A synthetic peptide from the internal region of mouse Ras-related protein Rab-5A (RAB5A) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat.
Marker
Early Endosome Marker
Specificity
RAB5A.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
RAB5A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:300-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1: 300- 1: 2000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24554775)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-20255 in the following applications:

Publications
Read Publication using
NBP1-20255 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Rab5a Antibody

  • RAB5A, member RAS oncogene family
  • RAB5RAS-associated protein RAB5A
  • ras-related protein Rab-5A

Background

FUNCTION: Required for the fusion of plasma membranes and early endosomes.; ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab5a Antibody (NBP1-20255)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Mouse
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Review for Rab5a Antibody (NBP1-20255) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-20255:
Filter by Applications
IF
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Rab5a NBP1-20255
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reviewed by:
Anonymous
IF Mouse 04/02/2015
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Summary

ApplicationImmunofluorescence
Sample Testedmouse bone marrow derived DCs
SpeciesMouse
LotNBP1-20255

Blocking

Blocking Details2%BSA 1h at RT

Primary Anitbody

Dilution Ratio1:200 dilution in 1XPS, incubated at 4degree overnight.

Secondary Antibody

Secondary DescriptionGoat anti-sheep IgG FITC conjugated
Secondary Concentration1:200

Details

Detection Notesusing fluorescent machine to scan the picture
Fixation Detailsmouse bone marrow derived DCs were seeded onto glass plate with cover, fixed, permeabilized and stained with this Ab
Wash Descriptionwash with PBS for 3 times

Comments

Commentsbackground is not clear enough, maybe due to high concentration

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab5a Antibody (NBP1-20255) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Rab5a

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/02/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol RAB5A
Entrez
Uniprot