RAB30 Antibody (OTI3E7)

Western Blot: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB30.

Immunocytochemistry/Immunofluorescence: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RAB30.

Immunohistochemistry: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of Human endometrium tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RAB30 Antibody (OTI3E7) [NBP2-45498] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RAB30 Antibody (OTI3E7) Summary

• Description: Novus Biologicals Mouse RAB30 Antibody (OTI3E7) (NBP2-45498) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RAB30 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RAB30(NP_055303) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB30
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200
• Theoretical MW: 22.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAB30 Antibody (OTI3E7)

• RAB30, member RAS oncogene family
• ras-related protein Rab-30

Background

RAB30( AAH14213, 1 a.a. - 204 a.a.) full-length recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAB30 Antibody (NBP2-45498)(1)

Publication using NBP2-45498

• Zhao L, Zhao J, Gao R, et al. SATB1 establishes ameloblast cell polarity and regulates directional amelogenin secretion for enamel formation BMC Biol. 2019-12-13 [PMID: 31830989] (WB, Human)

Bioinformatics

• Gene Symbol: RAB30