RAB22A Antibody (JE66-08)

Western Blot: RAB22A Antibody (JE66-08) [NBP3-32885] - Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (NBP3-32885) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: MCF7 cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: U-2 OS cell lysate (15 ug/Lane) Lane 7: A549 cell lysate (15 ug/Lane) Lane 8: HEK-293 cell lysate (15 ug/Lane) Lane 9: Mouse brain tissue lysate (30 ug/Lane) Lane 10: Rat brain tissue lysate (30 ug/Lane) Lane 11: Mouse kidney tissue lysate (30 ug/Lane) Lane 12: Mouse testis tissue lysate (30 ug/Lane) Lane 13: Rat liver testis lysate (30 ug/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32885) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: RAB22A Antibody (JE66-08) [NBP3-32885] - Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (NBP3-32885) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (NBP3-32885) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: JE66-08
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RAB22A Antibody (JE66-08) Summary

• Description: Novus Biologicals Rabbit RAB22A Antibody (JE66-08) (NBP3-32885) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human RAB22A aa 1-100 / 194. (Uniprot: Q9UL26)
• Localization: Endosome membrane, Cell membrane, Early endosome, Late endosome, Cell projection, ruffle, Cytoplasmic vesicle, Cytoplasmic vesicle, phagosome, phagosome membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB22A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RAB22A Antibody (JE66-08)

• GTP-binding protein RAB22A
• MGC16770
• RAB22
• Rab-22
• RAB22A, member RAS oncogene family
• ras-related protein Rab-22A

Background

RAB22A is encoded by this gene is a member of the RAB family of small GTPases. The GTP-bound form of the encoded protein has been shown to interact with early-endosomal antigen 1, and may be involved in the trafficking of and interaction between endosomal compartments. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB22A