RAB1B Antibody - BSA Free

Western Blot: RAB1B Antibody - BSA Free [NBP2-93131] - Western blot analysis of lysates from wild type (WT) and RAB1B knockout (KO) HeLa cells using [KO Validated] RAB1B Rabbit pAb RAB1B Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RAB1B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RAB1B Antibody - BSA Free (NBP2-93131) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1). YLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RAB1B Antibody - BSA Free

• RAB1B, member RAS oncogene family
• ras-related protein Rab-1B
• small GTP-binding protein

Background

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmicsurfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential forvesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 (PubMed 9030196); Alvarez etal., 2003 (PubMed 12802079)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB1B