GBF1 Antibody

Immunohistochemistry: GBF1 Antibody [NBP1-06526] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GBF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: GBF1 Antibody [NBP1-06526] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NBP1-06526 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. GBF1 was also immunoprecipitated by NBP1-06525 which recognizes an upstream epitope.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GBF1 Antibody Summary

• Description: Novus Biologicals Rabbit GBF1 Antibody (NBP1-06526) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1809 and 1859 of human golgi-specific brefeldin A resistance factor 1 using the numbering given in entry NP_004184.1 (GeneID 8729).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GBF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended.
• Theoretical MW: 206 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GBF1 Antibody

• ARF1GEF
• BFA-resistant GEF 1
• FLJ21263
• GBF1
• golgi brefeldin A resistant guanine nucleotide exchange factor 1
• golgi-specific brefeldin A resistance factor 1
• Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
• KIAA0248
• KIAA0248FLJ21500
• MGC134877
• MGC134878

Background

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GBF1
• Entrez:
  • 8729(Human)
  • 107338(Mouse)
• Uniprot:
  • Q92538(Human)