GBF1 Antibody (NBP1-06526)

Images

 
Immunohistochemistry: GBF1 Antibody [NBP1-06526] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GBF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunoprecipitation: GBF1 Antibody [NBP1-06526] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NBP1-06526 used for WB at 0.04 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

GBF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1809 and 1859 of human golgi-specific brefeldin A resistance factor 1 using the numbering given in entry NP_004184.1 (GeneID 8729).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GBF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended.
Theoretical MW
206 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GBF1 Antibody

  • ARF1GEF
  • BFA-resistant GEF 1
  • FLJ21263
  • golgi brefeldin A resistant guanine nucleotide exchange factor 1
  • golgi-specific brefeldin A resistance factor 1
  • Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
  • KIAA0248FLJ21500
  • MGC134877
  • MGC134878

Background

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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GBF1 NBP1-06526

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Bioinformatics

Gene Symbol GBF1
Entrez
Uniprot