R-Spondin 2 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

R-Spondin 2 Antibody Summary

• Immunogen: E. coli-derived recombinant human R-Spondin 2 isoform 1
  Cys21-Gly205
  Accession # Q6UXX9
• Specificity: Detects human R-Spondin 2 in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant human (rh) R-Spondin 1, rhR-Spondin 3, and recombinant mouse R-Spondin 4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RSPO2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 2 Antibody

• Cristin 2
• CRISTIN2
• hRspo2
• MGC35555
• MGC43342
• Roof plate-specific spondin-2
• RSPO2
• R-spondin 2 homolog (Xenopus laevis)
• RSpondin 2
• R-Spondin 2
• R-spondin-2

Background

Human Roof plate-specific Spondin 2 isoform 1 (R-Spondin 2, RSPO-2), also known as cysteine-rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family. The four known human R-Spondins regulate beta -catenin signaling and overlap in expression and function (1‑3). Like other R-Spondins, R-Spondin 2 contains two adjacent cysteine-rich furin-like domains (aa 90-134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207-243). The basic region appears to mediate cell surface retention but not to influence function (1). R‑Spondin 2 contains one potential N-glycosylation site. Of the three reported splice isoforms of human R-Spondin 2, isoform 2 lacks residues 1-67 of isoform 1, while isoform 3 has a glycine substitution for residues 32-95 of isoform 1. Human R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is downregulated in tumors of these tissues (1). In the embryonic mouse, R-Spondin 2 is expressed most highly in the hippocampus and in developing teeth and bones (4). Studies in Xenopus and mouse embryos indicate that R-Spondin 2 is an extracellular activator of Wnt/ beta -catenin signaling and is required for myogenesis (1). Mouse R-Spondin proteins bind both LRP-6 and Frizzled-8 but do not appear to form a ternary complex (3). R-Spondin 2 over-expression in Xenopus also blocks signaling of TGF-beta ligands, activin, nodal and BMP-4 (1). Human R-Spondin 2 is highly conserved across species, sharing 97-98% aa identity with mouse, rat, canine, bovine, and opossum R-Spondin 2 and 86% aa identity with Xenopus R-Spondin 2 within aa 22-205. Mature R-Spondin 2 shares ~40% aa identity with R-Spondin 1, R-Spondin 3, and R-Spondin 4.

1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
2. Kim, K-A. et al. (2006) Cell Cycle 5:23.
3. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
4. Nam, J-S. et al. (2007) Gene Expr. 281:13247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for R-Spondin 2 Antibody (AF3266)(3)

Publications using AF3266

• Guo, D;Pan, H;Lu, X;Chen, Z;Zhou, L;Chen, S;Huang, J;Liang, X;Xiao, Z;Zeng, H;Shao, Y;Qi, W;Xie, D;Lin, C; Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/?-catenin pathway Arthritis research & therapy 2023-11-09 [PMID: 37946278] (Bioassay, Human)
• R Sun, L He, H Lee, A Glinka, C Andresen, D Hübschmann, I Jeremias, K Müller-Dec, C Pabst, C Niehrs RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemia Cell Reports, 2021-08-17;36(7):109559. 2021-08-17 [PMID: 34407399] (Neutralization, Human)
• Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring. J. Invest. Dermatol., 2010-12-16;131(3):644-54. 2010-12-16 [PMID: 21160497] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RSPO2
• Uniprot:
  • Q6UXX9()