Recombinant Human R-Spondin 3 Protein

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Recombinant Human R-Spondin 3 (Catalog # 3500-RS), in the presence of Recombinant Mouse Wnt 3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human R-Spondin 3 Protein Summary

Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.5‑2.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 3 protein
Gln22-His272
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
RSPO3
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36-45 kDa, reducing conditions
Publications
Read Publications using
3500-RS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% of human or bovine serun albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human R-Spondin 3 Protein

  • Cristin 1
  • CRISTIN1
  • FLJ14440
  • hPWTSR
  • hRspo3
  • Protein with TSP type-1 repeat
  • PWTSR
  • Roof plate-specific spondin-3
  • RSPO3
  • R-spondin 3 homolog (Xenopus laevis)
  • RSpondin 3
  • R-Spondin 3
  • R-spondin-3
  • Thrombospondin type-1 domain-containing protein 2
  • thrombospondin, type I, domain containing 2
  • THSD2

Background

R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% amino acid (aa) identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1-4). Like other R-spondins,R-Spondin 3 contains two adjacent cysteine-rich furin-like domains (aa 35-135) with one potential N-glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21-209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta-specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8-10). R-Spondin 3 has also been identified as an oncogene (11).

  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  5. Entrez Accession # EAW48114 and EAW48116.
  6. Kazanskaya, O. et al. (2008) Development 135:3655.
  7. Aoki, M. et al. (2007) Dev. Biol. 301:218.
  8. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  9. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  10. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  11. Theodorou, V. et al. (2007) Nat. Genet. 6:759.

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3500-RS
Species: Hu
Applications: Bioactivity

Publications for R-Spondin 3 (3500-RS)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey).

We have publications tested in 2 applications: Bioassay, Cell Culture.


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Bioassay
(6)
Cell Culture
(2)
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Human
(6)
Mouse
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
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Showing Publications 1 - 9 of 9.
Publications using 3500-RS Applications Species
PG Boone, LK Rochelle, JD Ginzel, V Lubkov, WL Roberts, PJ Nicholls, C Bock, ML Flowers, RJ von Furste, BR Stripp, P Agarwal, AD Borowsky, RD Cardiff, LS Barak, MG Caron, HK Lyerly, JC Snyder A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion Nat Commun, 2019;10(1):5490. 2019 [PMID: 31792216] (Cell Culture, Human) Cell Culture Human
S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29752411] (Bioassay, Human) Bioassay Human
AM Lebensohn, R Rohatgi R-spondins can potentiate WNT signaling without LGRs Elife, 2018;7(0):. 2018 [PMID: 29405118] (Bioassay, Human) Bioassay Human
D Zeve, DP Millay, J Seo, JM Graff Exercise-Induced Skeletal Muscle Adaptations Alter the Activity of Adipose Progenitor Cells PLoS ONE, 2016;11(3):e0152129. 2016 [PMID: 27015423] (Bioassay, Primate - Chlorocebus pygerythrus (Vervet Monkey)) Bioassay Primate - Chlorocebus pygerythrus (Vervet Monkey)
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res, 2016;76(3):713-23. 2016 [PMID: 26719531] (Bioassay, Mouse) Bioassay Mouse
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun, 2014;5(0):3149. 2014 [PMID: 24476626]
Xie, Yang, Zamponi, Raffaell, Charlat, Olga, Ramones, Melissa, Swalley, Susanne, Jiang, Xiaomo, Rivera, Daniel, Tschantz, William, Lu, Bo, Quinn, Lisa, Dimitri, Chris, Parker, Jefferso, Jeffery, Doug, Wilcox, Sheri K, Watrobka, Mike, LeMotte, Peter, Granda, Brian, Porter, Jeffrey, Myer, Vic E, Loew, Andreas, Cong, Feng Interaction with both ZNRF3 and LGR4 is required for the signalling activity of R-spondin. EMBO Rep, 2013;14(12):1120-6. 2013 [PMID: 24165923] (Cell Culture, Human) Cell Culture Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature, 2012;485(7397):195-200. 2012 [PMID: 22575959] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol RSPO3
Uniprot