R-Spondin 2 Antibody (CL14321) - Azide and BSA Free

Analysis in human placenta and skeletal muscle tissues using NBP3-44331 antibody. Corresponding R-Spondin 2 RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.

Staining of human ovary shows weak to moderate cytoplasmic positivity in oocytes.

Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of mouse brain shows moderate to strong cytoplasmic positivity in neurons.

• Reactivity: Hu, Mu
• Applications: IHC
• Clone: CL14321
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

R-Spondin 2 Antibody (CL14321) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse R-Spondin 2 Antibody (CL14321) - Azide and BSA Free (NBP3-26851) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q6UXX9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RSPO2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for R-Spondin 2 Antibody (CL14321) - Azide and BSA Free

• Cristin 2
• CRISTIN2
• hRspo2
• MGC35555
• MGC43342
• Roof plate-specific spondin-2
• RSPO2
• R-spondin 2 homolog (Xenopus laevis)
• RSpondin 2
• R-Spondin 2
• R-spondin-2

Background

R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RSPO2