R-Spondin 2 Antibody (879715R) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
879715R
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

R-Spondin 2 Antibody (879715R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line NS0-derived recombinant human R‑Spondin 2
Met1-Gly205
Accession # Q6UXX9
Specificity
Detects human R-Spondin 2 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 2 Antibody (879715R) [Unconjugated]

  • Cristin 2
  • CRISTIN2
  • hRspo2
  • MGC35555
  • MGC43342
  • Roof plate-specific spondin-2
  • RSPO2
  • R-spondin 2 homolog (Xenopus laevis)
  • RSpondin 2
  • R-Spondin 2
  • R-spondin-2

Background

Human Roof plate-specific Spondin 2 isoform 1 (R-Spondin 2, RSPO-2), also known as cysteine-rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family. The four known human R-Spondins regulate beta -catenin signaling and overlap in expression and function (1‑3). Like other R-Spondins, R-Spondin 2 contains two adjacent cysteine-rich furin-like domains (aa 90-134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207-243). The basic region appears to mediate cell surface retention but not to influence function (1). R‑Spondin 2 contains one potential N-glycosylation site. Of the three reported splice isoforms of human R-Spondin 2, isoform 2 lacks residues 1-67 of isoform 1, while isoform 3 has a glycine substitution for residues 32-95 of isoform 1. Human R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is downregulated in tumors of these tissues (1). In the embryonic mouse, R-Spondin 2 is expressed most highly in the hippocampus and in developing teeth and bones (4). Studies in Xenopus and mouse embryos indicate that R-Spondin 2 is an extracellular activator of Wnt/ beta -catenin signaling and is required for myogenesis (1). Mouse R-Spondin proteins bind both LRP-6 and Frizzled-8 but do not appear to form a ternary complex (3). R-Spondin 2 over-expression in Xenopus also blocks signaling of TGF-beta ligands, activin, nodal and BMP-4 (1). Human R-Spondin 2 is highly conserved across species, sharing 97-98% aa identity with mouse, rat, canine, bovine, and opossum R-Spondin 2 and 86% aa identity with Xenopus R-Spondin 2 within aa 22-205. Mature R-Spondin 2 shares ~40% aa identity with R-Spondin 1, R-Spondin 3, and R-Spondin 4.

  1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  2. Kim, K-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
  4. Nam, J-S. et al. (2007) Gene Expr. 281:13247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

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