R-Spondin 1 Antibody (7A6)

Western Blot: R-Spondin 1 Antibody (7A6) [NBP1-28867] - analysis using anti-R-spondin1 monoclonal antibody against recombinant R-spondin1 protein(1) and R-spondin1(aa21-263)-hIgGFc transfected HEK293 cell lysate(2).

Product Discontinued

R-Spondin 1 Antibody (7A6) Summary

• Immunogen: Purified recombinant fragment of R-spondin1 expressed in E. Coli.
• Specificity: Specific for R-spondin1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RSPO1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000
• Theoretical MW: 28.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for R-Spondin 1 Antibody (7A6)

• Cristin 3
• CRISTIN3
• FLJ40906Roof plate-specific spondin-1
• HRspo1
• roof plate-specific spondin
• RSPO
• RSPO1
• RSpondin 1
• R-Spondin 1
• R-spondin homolog (Xenopus laevis)
• RSPONDIN
• R-spondin1
• R-spondin-1

Background

R-spondin1, also known as RSPO1, CRISTIN3, FLJ40906. Entrez Protein NP_001033722.1. It is a member of the R-spondin family and a 28.9kDa secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.It is activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RSPO1