Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric)

ELISA: Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric) [NBP2-81238] - Samples were spiked with high concentrations of Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric) [NBP2-81238] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 3.13 - 200 mIU/mL
• Sensitivity: 1.88 mIU/mL

Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C in samples. No significant cross-reactivity or interference between Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C and analogues was observed.
• Standard Curve Range: 3.13 - 200 mIU/mL
• Sensitivity: 1.88 mIU/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.89%
• Intra-Assay: CV% < 5.39%
• Spike Recovery: 87-110%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PDP1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric)

• [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
• EC 3.1.3
• EC 3.1.3.43
• MGC119646
• PDH
• PDP 1
• PDP1
• PDPC 1
• PDPC
• PDPFLJ32517
• PPM2C
• PPM2CFLJ56179
• Protein phosphatase 2C
• protein phosphatase 2C, magnesium-dependent, catalytic subunit
• pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase
• Pyruvate dehydrogenase phosphatase catalytic subunit 1
• Pyruvate Dehydrogenase Phosphatase
• pyruvate dehyrogenase phosphatase catalytic subunit 1

Background

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (NBP2-81238). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PDP1