PYCRL Antibody (4F11) - Azide and BSA Free

Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - PYCRL monoclonal antibody (M01), clone 4F11 Analysis of PYCRL expression in HeLa.

Immunocytochemistry/Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of monoclonal antibody to PYCRL on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL expression in transfected 293T cell line by PYCRL monoclonal antibody (M01), clone 4F11.Lane 1: PYCRL transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL transfected lysate using anti-PYCRL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PYCRL monoclonal antibody.

ELISA: PYCRL Antibody (4F11) [H00065263-M01] - Detection limit for recombinant GST tagged PYCRL is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 4F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PYCRL Antibody (4F11) - Azide and BSA Free Summary

• Immunogen: PYCRL (NP_075566, 32 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVV
• Specificity: PYCRL (4F11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PYCR3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PYCRL Antibody (4F11) - Azide and BSA Free

• EC 1.5.1.2
• FLJ13852
• P5C reductase 3
• P5CR 3
• pyrroline-5-carboxylate reductase 3
• Pyrroline-5-carboxylate reductase-like protein
• pyrroline-5-carboxylate reductase-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PYCRL Antibody (H00065263-M01)(4)

Publications using H00065263-M01

• Hamanaka, RB;O'Leary, EM;Witt, LJ;Tian, Y;Gokalp, GA;Meliton, AY;Dulin, NO;Mutlu, GM; Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts Am. J. Respir. Cell Mol. Biol. 2019-04-11 [PMID: 30973753] (WB, Human)
• Haider S, McIntyre A, van Stiphout RG et al. Genomic alterations underlie a pan-cancer metabolic shift associated with tumour hypoxia. Genome Biol 2016-06-29 [PMID: 27358048] (WB)
• Zaki MS, Bhat G, Sultan T et al. PYCR2 mutations cause a lethal syndrome of microcephaly and failure to thrive. Ann. Neurol. 2016-04-30 [PMID: 27130255] (WB, Human)
• De Ingeniis J, Ratnikov B, Richardson AD et al. Functional specialization in proline biosynthesis of melanoma. PLoS One. 2012-01-01 [PMID: 23024808] (Human)

Bioinformatics

• Gene Symbol: PYCR3
• Entrez:
  • 65263(Human)
• Uniprot:
  • NP_075566(Human)