Recombinant Human PYCR1 Protein

Images

 
SDS-Page: Recombinant Human PYCR1 Protein [NBP1-50895] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human PYCR1 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-319 of Human PYCR1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD

Details of Functionality
Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PYCR1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl Buffer (pH 8.5) containing 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PYCR1 Protein

  • ARCL2B
  • EC 1.5.1.2
  • mitochondrial pyrroline-5-carboxylate reductase 1
  • P5C reductase 1
  • P5C
  • P5CR 1
  • P5CR
  • PIG45
  • PP222
  • PRO3
  • proliferation-inducing protein 45
  • PYCR
  • pyrroline-5-carboxylate reductase 1
  • pyrroline-5-carboxylate reductase 1, mitochondrial

Background

PYCR1 is a universal housekeeping enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-32390
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2832
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
H00008659-D01P
Species: Hu, Mu
Applications: WB
NBP1-83324
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF4885
Species: Mu
Applications: WB, IP
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-00092
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-Fr, PLA
NBP1-59069
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45912
Species: Hu
Applications: WB, IHC
NBP2-30783
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-44374
Species: Hu
Applications: Flow, IHC-P, IF
H00005545-R02
Species: Hu
NBP2-45897
Species: Hu
Applications: WB, IHC
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-25151
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P

Publications for PYCR1 Protein (NBP1-50895) (0)

There are no publications for PYCR1 Protein (NBP1-50895).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PYCR1 Protein (NBP1-50895) (0)

There are no reviews for PYCR1 Protein (NBP1-50895). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for PYCR1 Protein (NBP1-50895): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PYCR1 Protein (NBP1-50895) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PYCR1 Products

Bioinformatics Tool for PYCR1 Protein (NBP1-50895)

Discover related pathways, diseases and genes to PYCR1 Protein (NBP1-50895). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PYCR1 Protein (NBP1-50895)

Discover more about diseases related to PYCR1 Protein (NBP1-50895).
 

Pathways for PYCR1 Protein (NBP1-50895)

View related products by pathway.

PTMs for PYCR1 Protein (NBP1-50895)

Learn more about PTMs related to PYCR1 Protein (NBP1-50895).

Blogs on PYCR1

There are no specific blogs for PYCR1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PYCR1 Antibody
NBP2-20016

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human PYCR1 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol PYCR1