Recombinant Human PTRH1 Protein

Product Discontinued

Recombinant Human PTRH1 Protein Summary

• Description: Recombinant biologically active PRTH1 protein.

  Source:E. coli

  Amino Acid Sequence: Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe

• Details of Functionality: rHuPTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0 x 10^4 Units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PTRH1
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) of rHuPTH.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 4.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized after extensive dialyses against sodium citrate containing sodium chloride, citric acid, EDTA-Na2, Tween 80 and Mannitol.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Reconstitute with sterilized 18 M omega-cm water to a final concentration of at least 0.1 mg/ml.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Human PTRH1 Protein

• C9orf115
• chromosome 9 open reading frame 115
• EC 3.1.1.29
• MGC51999
• peptidyl-tRNA hydrolase 1 homolog (S. cerevisiae)
• probable peptidyl-tRNA hydrolase
• PTH
• PTH1

Background

Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calciumin the blood, whereas calcitonin (a hormone produced by the parafollicular cells of the thyroid gland) acts to decrease calcium concentration. PTH acts to increase the concentration of calcium in the blood by acting upon parathyroid hormone receptorin three parts of the body: In the bones- It enhances the release of calcium from the large reservoir contained in the bones. Bone resorption is the normal destruction of bone by osteoclasts, which are indirectly stimulated by PTH. Stimulation is indirect since osteoclasts do not have a receptor for PTH; rather, PTH binds to osteoblasts, the cells responsible for creating bone. Binding stimulates osteoblasts to increase their expression of RANKL, which can bind to osteoclast precursors containing RANK, a receptor for RANKL. The binding of RANKL to RANK stimulates these precursors to fuse, forming new osteoclasts which ultimately enhances the resorption of bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PTRH1