PTPRCAP Overexpression Lysate

Images

 
Western Blot: PTPRCAP Overexpression Lysate (Adult Normal) [NBL1-14982] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPRCAP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PTPRCAP Overexpression Lysate Summary

Description

PTPRCAP Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC224803

Accession#: NM_005608

Protein Tag: C-MYC/DDK
Gene
PTPRCAP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PTPRCAP Overexpression Lysate

  • CD45-APCD45 associated protein
  • CD45-associated protein
  • LPAPMGC138603
  • Lymphocyte phosphatase-associated phosphoprotein
  • MGC138602
  • protein tyrosine phosphatase receptor type C-associated protein
  • protein tyrosine phosphatase, receptor type, c polypeptide-associated protein
  • protein tyrosine phosphatase, receptor type, C-associated protein
  • PTPRC-associated protein

Background

The protein encoded by this gene was identified as a transmembrane phosphoprotein specifically associated with tyrosine phosphatase PTPRC/CD45, a key regulator of T- and B-lymphocyte activation. The interaction with PTPRC may be required for the stable expression of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PTPRCAP