PTPRCAP Overexpression Lysate (Native)

Images

 
Western Blot: PTPRCAP Overexpression Lysate (Adult Normal) [NBL1-14982] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPRCAP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PTPRCAP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224803 and based on accession number NM_005608. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein tyrosine phosphatase, receptor type, C-associated protein (PTPRCAP), mRNA.
Gene
PTPRCAP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PTPRCAP Overexpression Lysate (Native)

  • CD45-APCD45 associated protein
  • CD45-associated protein
  • LPAPMGC138603
  • Lymphocyte phosphatase-associated phosphoprotein
  • MGC138602
  • protein tyrosine phosphatase receptor type C-associated protein
  • protein tyrosine phosphatase, receptor type, c polypeptide-associated protein
  • protein tyrosine phosphatase, receptor type, C-associated protein
  • PTPRC-associated protein

Background

The protein encoded by this gene was identified as a transmembrane phosphoprotein specifically associated with tyrosine phosphatase PTPRC/CD45, a key regulator of T- and B-lymphocyte activation. The interaction with PTPRC may be required for the stable expression of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PTPRCAP