PTPRB Antibody (2-A2)

Immunocytochemistry/Immunofluorescence: PTPRB Antibody (2-A2) [NBP2-67087] - Staining PTPRB in F9 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

Product Discontinued

PTPRB Antibody (2-A2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human PTPRB aa 1,018-1,161 / 1,997. (SwissProt: P23467 Human)
• Localization: Membrane
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for PTPRB Antibody (2-A2)

• DKFZp686E2262
• DKFZp686H15164
• EC 3.1.3.48
• FLJ44133
• HPTPB
• HPTP-BETA
• MGC142023
• MGC59935
• protein tyrosine phosphatase, receptor type, B
• protein tyrosine phosphatase, receptor type, beta polypeptide
• Protein-tyrosine phosphatase beta
• PTPB
• receptor-type tyrosine-protein phosphatase beta
• R-PTP-BETA
• Vascular endothelial protein tyrosine phosphatase
• VEPTP
• VE-PTP

Background

PTPRB is a member of the protein tyrosine phosphatase (PTP) family. The family comprises at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is a transmembrane PTP. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP beta interacts with and regulates the tyrosine phosphorylation level of catenins, which are critical in physiological and pathological events such as cell migration, adhesion and transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRB