PTPN12 Antibody - BSA Free

Western Blot: PTPN12 Antibody [NBP2-20004] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: PTPN12 Antibody [NBP2-20004] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP2-20004] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP2-20004] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP2-20004] - Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using PTPN12 antibody.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PTPN12 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PTPN12. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN12
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus tropicalis (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PTPN12 Antibody - BSA Free

• protein tyrosine phosphatase, non-receptor type 12
• Protein-tyrosine phosphatase G1
• PTPG1
• PTPG1tyrosine-protein phosphatase non-receptor type 12
• PTPN12
• PTP-PEST
• PTP-PESTEC 3.1.3.48

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PTPN12
• Uniprot:
  • Q05209(Human)