PTPN12 Antibody - BSA Free

Western Blot: PTPN12 Antibody [NBP1-87188] - Analysis in human cell line U-251 MG.

Immunocytochemistry/Immunofluorescence: PTPN12 Antibody [NBP1-87188] - Staining of human cell line U-251 MG shows positivity in plasma membrane and cytoplasm.

Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP1-87188] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PTPN12 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SVTQSNKVSVTPPEESQNSDTPPRPDRLPLDEKGHVTWSFHGPENAIPIPDLSEGNSSDINYQTRKTVSLTPSPTTQVETPDLVDHDNTSPLFRTPLSFTNPLHSDDSDSDERNSDGAVTQNKTNISTASATVSAAT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: IHC, WB reported in scientific literature (PMID: 25868976). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PTPN12 Recombinant Protein Antigen (NBP1-87188PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435421)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PTPN12 Antibody - BSA Free

• protein tyrosine phosphatase, non-receptor type 12
• Protein-tyrosine phosphatase G1
• PTPG1
• PTPG1tyrosine-protein phosphatase non-receptor type 12
• PTPN12
• PTP-PEST
• PTP-PESTEC 3.1.3.48

Background

PTPN12 is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTPN12 Antibody (NBP1-87188)(2)

Publications using NBP1-87188

• Cao X, Chen YZ, Luo RZ et al. Tyrosine-protein phosphatase non-receptor type 12 expression is a good prognostic factor in resectable non-small cell lung cancer. Oncotarget 2015-05-10 [PMID: 25868976] (WB, IF/IHC, Human)
• Harris IS, Blaser H, Moreno J et al. PTPN12 promotes resistance to oxidative stress and supports tumorigenesis by regulating FOXO signaling. Oncogene 2014-02-20 [PMID: 23435421]

Bioinformatics

• Gene Symbol: PTPN12