PTPN12 Antibody (BL-5-2F8)

Western Blot: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 [SK], and HCT 116 lysate. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunocytochemistry/Immunofluorescence: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 in FFPE SW620 cells by ICC. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 in FFPE non-small cell lung carcinoma by IHC. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 in FFPE mammary gland by IHC. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395) and another rabbit anti-PTPN12 antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: BL-5-2F8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PTPN12 Antibody (BL-5-2F8) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Immunogen sequence between amino acids 499 and 780
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PTPN12
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Immunoprecipitation 20 ul/0.5 mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for PTPN12 Antibody (BL-5-2F8)

• protein tyrosine phosphatase, non-receptor type 12
• Protein-tyrosine phosphatase G1
• PTPG1
• PTPG1tyrosine-protein phosphatase non-receptor type 12
• PTPN12
• PTP-PEST
• PTP-PESTEC 3.1.3.48

Background

PTPN12 is a cytosolic protein tyrosine phosphatase (PTP) that contains a C-terminal PEST motif and plays an important role in cytoskeletal organization, immune cell activation, apoptosis, and embryonic development. PTPN12 appears to contribute to these cellular processes through its interaction with a variety of signaling and cytoskeletal-associated proteins. The recent discovery that PTPN12 is modulated by caspase-3 cleavage has led to the analysis of the role of PTPN12 and other PTPs as regulators of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN12