| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P |
| Clone | SPM604 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 755 |
| Immunogen | A synthetic peptide from the N-terminal of human PTH polypeptide. |
| Localization | Cytoplasmic and Secreted |
| Specificity | Epitope of this MAb maps in the N-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTH |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![PTH Antibody (SPM604) [DyLight 755]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "PTH Antibody (SPM604) [DyLight 755]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54434AF350 | |
| Alexa Fluor 405 | NBP2-54434AF405 | |
| Alexa Fluor 488 | NBP2-54434AF488 | |
| Alexa Fluor 532 | NBP2-54434AF532 | |
| Alexa Fluor 594 | NBP2-54434AF594 | |
| Alexa Fluor 647 | NBP2-54434AF647 | |
| Alexa Fluor 700 | NBP2-54434AF700 | |
| Alexa Fluor 750 | NBP2-54434AF750 | |
| Allophycocyanin | NBP2-54434APC | |
| Biotin | NBP2-54434B | |
| DyLight 350 | NBP2-54434UV | |
| DyLight 405 | NBP2-54434V | |
| DyLight 488 | NBP2-54434G | |
| DyLight 550 | NBP2-54434R | |
| DyLight 594 | NBP2-54434DL594 | |
| DyLight 650 | NBP2-54434C | |
| DyLight 680 | NBP2-54434FR | |
| DyLight 755 | NBP2-54434IR | |
| FITC | NBP2-54434F | |
| HRP | NBP2-54434H | |
| Janelia Fluor 549 | NBP2-54434JF549 | |
| Janelia Fluor 646 | NBP2-54434JF646 | |
| PE | NBP2-54434PE | |
| PerCP | NBP2-54434PCP |
Diseases for PTH Antibody (NBP2-54434IR)Discover more about diseases related to PTH Antibody (NBP2-54434IR).
| Pathways for PTH Antibody (NBP2-54434IR)View related products by pathway.
|
PTMs for PTH Antibody (NBP2-54434IR)Learn more about PTMs related to PTH Antibody (NBP2-54434IR).
| Research Areas for PTH Antibody (NBP2-54434IR)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PTH |
![Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - HepG2 cell lysate, Antibody Titration: 2.0ug/ml](http://images.novusbio.com/images2/PTHLH-PTHrP-Antibody-Western-Blot-NBP1-59322-img0001.jpg)
![Immunohistochemistry-Paraffin: PTHLH/PTHrP Antibody [NBP1-59322] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.](http://images.novusbio.com/images2/PTHLH-PTHrP-Antibody-Immunohistochemistry-Paraffin-NBP1-59322-img0003.jpg)
![Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary, Antibody Dilution: 1 : 2000.](http://images.novusbio.com/images2/PTHLH-PTHrP Antibody-Western Blot-NBP1-59322-img0004.jpg)
![Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) [NB300-164] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm.](http://images.novusbio.com/images2/Bestrophin-1-Antibody-(E6-6)-Dual-RNAscope-ISH-IHC-NB300-164-img0006.jpg)
![Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and extracellular.](http://images.novusbio.com/images2/Bestrophin-1-Antibody-(E6-6)-Immunohistochemistry-Paraffin-NB300-164-img0005.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) [NB300-164] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Bestrophin-1-Antibody-(E6-6)-Western-Blot-NB300-164-img0007.jpg)
![Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining in human skeletal muscle and tonsil tissues. Corresponding DMD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Dystrophin-Antibody-Immunohistochemistry-Paraffin-NBP1-89953-img0010.jpg)
![Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human heart muscle shows strong membranous positivity in myocytes.](http://images.novusbio.com/images2/Dystrophin-Antibody-Immunohistochemistry-Paraffin-NBP1-89953-img0007.jpg)
![Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human skeletal muscle shows strong membranous positivity in myocytes.](http://images.novusbio.com/images2/Dystrophin-Antibody-Immunohistochemistry-Paraffin-NBP1-89953-img0008.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CaSR in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/images2/Calcium-sensing-R-CaSR-Antibody-(5C10--ADD)-Western-Blot-NB120-19347-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of bovine corneal epithelium (CE) limbus tissue.](http://images.novusbio.com/images2/Calcium-sensing-R-CaSR-Antibody-(5C10--ADD)-Immunocytochemistry-Immunofluorescence-NB120-19347-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.](http://images.novusbio.com/images2/Calcium-sensing-R-CaSR-Antibody-(5C10--ADD)-Immunohistochemistry-Paraffin-NB120-19347-img0011.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Analysis of anti-PTH1R antibody with human liver at concentration 5 ug/ml.](http://images.novusbio.com/images2/PTH1R-PTHR1-Antibody-(3D1.1)-Immunohistochemistry-Paraffin-NBP1-40067-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Alkaline-Phosphatase-ALPP-Antibody-(8B6)-Immunocytochemistry-Immunofluorescence-NB110-3638-img0005.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used.](http://images.novusbio.com/images2/Alkaline-Phosphatase--Placental-Antibody-(8B6)-Immunohistochemistry-Paraffin-NB110-3638-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.](http://images.novusbio.com/images2/Alkaline-Phosphatase-ALPP-Antibody-(8B6)-Immunocytochemistry-Immunofluorescence-NB110-3638-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATRNL1 Antibody [NBP1-89131] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/images2/ATRNL1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89131-img0005.jpg)
![Immunohistochemistry-Paraffin: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.](http://images.novusbio.com/images2/ATRNL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89131-img0004.jpg)
![Immunohistochemistry: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.](http://images.novusbio.com/images2/ATRNL1-Antibody-Immunohistochemistry-NBP1-89131-img0002.jpg)
![Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/ASRGL1-Antibody-Western-Blot-NBP1-89133-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.](http://images.novusbio.com/images2/ASRGL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89133-img0004.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human cervix, uterine, endometrium, fallopian tube and skeletal muscle using Anti-ASRGL1 antibody NBP1-89133 (A) shows similar protein distribution across tissues to independent antibody NBP2-38759 (B).](http://images.novusbio.com/images2/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89133-img0012.jpg)
![Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining in human skeletal muscle and cerebral cortex tissues using anti-PDLIM3 antibody. Corresponding PDLIM3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/PDLIM3-Antibody-Immunohistochemistry-Paraffin-NBP1-89134-img0008.jpg)
![Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/PDLIM3-Antibody-Immunohistochemistry-Paraffin-NBP1-89134-img0006.jpg)
![Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/images2/PDLIM3-Antibody-Immunohistochemistry-Paraffin-NBP1-89134-img0007.jpg)
