PSPC1 Antibody - BSA Free

Western Blot: PSPC1 Antibody [NBP3-12643] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: PSPC1 Antibody [NBP3-12643] - Staining of human PSPC1 in human testis with rabbit polyclonal antibody at 1:2000 dilution.

Immunoprecipitation: PSPC1 Antibody [NBP3-12643] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PSPC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSPC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size :72 kDa

Immunocytochemistry/Immunofluorescence: PSPC1 Antibody [NBP3-12643] - Staining of PSPC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12643 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSPC1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PSPC1 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSPC1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:500-1:3000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PSPC1 Antibody - BSA Free

• FLJ33554
• paraspeckle component 1
• paraspeckle protein 1
• PSP1

Background

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line . Binds to poly(A), poly(G) and poly(U) RNA homopolymers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSPC1