PSMC3 Antibody - BSA Free

Western Blot: PSMC3 Antibody [NBP1-32749] - (30 ug of whole cell lysate) A: MOLT4 10% SDS PAGE diluted at 1:5000

Immunocytochemistry/Immunofluorescence: PSMC3 Antibody [NBP1-32749] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PSMC3 Antibody [NBP1-32749] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry: PSMC3 Antibody [NBP1-32749] - Immunofluorescent image of a zebrafish embryo eye section using PSMC3 antibody [N1C2] (NBP1-32749) at a 1:200 dilution. Psmc3 (Green) Actin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSMC3 antibody [N1C2] (NBP1-32749) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSMC3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PSMC3 Antibody - BSA Free (NBP1-32749) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PSMC3. The exact sequence is proprietary.
• Localization: Cytoplasm (Potential), Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMC3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PSMC3 Antibody - BSA Free

• human immunodeficiency virus tat transactivator binding protein-1
• MGC8487
• proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5
• Proteasome 26S subunit ATPase 3
• Proteasome subunit P50
• TBP-126S protease regulatory subunit 6A
• TBP1Tat-binding protein 1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PSMC3