PSMA1 Antibody (JB89-30)

Western Blot: PSMA1 Antibody (JB89-30) [NBP2-75628] - Analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution.Positive control:Lane 1: PC-3M Lane 2: K562 Lane 3: Rat testisLane 4: Mouse testis Lane 5: Mouse colon

Immunohistochemistry-Paraffin: PSMA1 Antibody (JB89-30) [NBP2-75628] - Analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

Flow Cytometry: PSMA1 Antibody (JB89-30) [NBP2-75628] - Analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: PSMA1 Antibody (JB89-30) [NBP2-75628] - Analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PSMA1 Antibody (JB89-30) [NBP2-75628] - Analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PSMA1 Antibody (JB89-30) [NBP2-75628] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

Product Discontinued

PSMA1 Antibody (JB89-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PSMA1 aa 214-263 / 263. (SwissProt: P25786 Human; SwissProt: Q9R1P4 Mouse; SwissProt: P18420 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PSMA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PSMA1 Antibody (JB89-30)

• HC2;HEL-S-275;NU;PROS30;Proteasome subunit alpha type;Proteasome subunit alpha type-1
• Macropain Subunit C2
• P30-33K
• PROS30
• Proteasome Component C2
• Proteasome nu Chain
• PSC2
• PSMA1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMA1