PSMA/FOLH1/NAALADase I Antibody (GCP-05) - BSA Free

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [NBP1-45058] - Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [PE] [NBP1-45056] - Surface staining (flow cytometry) of GCPII / PSMA using anti-GCPII (GCP-05) PE on LNCaP cell line. Image using the PE form of this antibody.

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [NBP1-45058] - Analysis of GCPII (PSMA) by various monoclonal antibodies (GCP-01, GCP-02, GCP-04, GCP-05) on LNCaP cell line. Legend: Green column - 1 ug/ml, LNCaP cell line Blue column - 5 ug/ml, LNCaP cell line Yellow column - negative cell line

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [NBP1-45058] - Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [PE] [NBP1-45056] - Surface staining (flow cytometry) of GCPII / PSMA using anti-GCPII (GCP-05) Alexa Fluor 488 on LNCaP cell line. Image using the PE form of this antibody.

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [NBP1-45058] - Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (GCP-05) [PE] [NBP1-45056] - Surface staining (mass cytometry) of LNCaP cell line using anti-GCPII (GCP-05) 173Yb. Gated on singlets. Image using the PE form of this antibody.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IP, CyTOF-ready
• Clone: GCP-05
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PSMA/FOLH1/NAALADase I Antibody (GCP-05) - BSA Free Summary

• Immunogen: amino acids 44-750 of human GCPII
• Specificity: The antibody GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (GCP-05) - BSA Free

• Cell growth-inhibiting gene 27 protein
• cell growth-inhibiting protein 27
• EC 3.4.17.21
• FGCP
• folate hydrolase (prostate-specific membrane antigen) 1
• Folate hydrolase 1
• FOLH1
• FOLHNAALADase I
• Folylpoly-gamma-glutamate carboxypeptidase
• GCP2
• GCPII
• GCPIImGCP
• glutamate carboxylase II
• glutamate carboxypeptidase 2
• Glutamate carboxypeptidase II
• Membrane glutamate carboxypeptidase
• mopsm
• NAALAD1
• NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
• NAALADase I
• NAALAdase
• N-acetylated alpha-linked acidic dipeptidase 1
• prostate specific membrane antigen variant F
• Prostate-specific membrane antigen
• PSMA
• PSMAFGCP
• PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer's disease, Huntington's disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM') than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PSMA/FOLH1/NAALADase I Antibody (NBP1-45058). (Showing 1 - 1 of 1 FAQ).

1. Hi, I was wondering if this antibody: NBP1-45058 is suitable for extracellular immunolabeling.
   • Here is the specificity information:The antibody GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.

Bioinformatics

• Gene Symbol: FOLH1