PSCA Antibody

Product Discontinued

PSCA Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human PSCA.
• Marker: Prostate Stem Cell Marker
• Specificity: Prostate Stem Cell Antigen (V71) detects endogenous levels of Prostate Stem Cell Antigen protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSCA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Prostate Stem Cell Antigen (V71) pAb in extracts from HepG2 cells. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSCA Antibody

• PRO232
• prostate stem cell antigen
• PSCA

Background

Prostate stem cell antigen (PSCA) is a 123 amino acid glycosylated protein that shares homology with the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol (GPI)-anchored cell surface antigens. The PSCA gene maps to chromosome 8q24.2 and transcripts are most prevalent in prostate and placenta. The gene encoding c-myc is also located on chromosome 8q and like PSCA, is overexpressed in a large number of prostate cancers. Transcripts for PSCA are also abundant in urothelial tumors, and levels of PSCA transcripts increase in confluent RT112 bladder carcinomas, suggesting that PSCA is a marker for urothelial and gastric tissue carcinogenesis. Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and correlates well to certain prostate cancer phenotypes such as prostate cancer bone metastates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSCA Antibody (NB100-91938)(1)

Publication using NB100-91938

• Tanikawa C, Urabe Y, Matsuo K,et al. A genome-wide association study identifies two susceptibility loci for duodenal ulcer in the Japanese population Nat Genet 2012-03-04 [PMID: 22387998] (WB, ICC/IF, Human)

FAQs for PSCA Antibody (NB100-91938). (Showing 1 - 1 of 1 FAQ).

1. I would like to verify our staining with your PSCA antibody (NB100-91938). So my question is: was the peptide with cat. no. H00008000-Q01 used to generate the antibody?
   • The immunogen for NB100-91938 is a synthetic peptide corresponding to the residues surrounding Valine 71 of human Prostate Stem Cell Antigen. It is not however peptide, H00008000-Q01.

Bioinformatics

• Gene Symbol: PSCA
• Entrez:
  • 8000(Human)
  • 72373(Mouse)
• Uniprot:
  • O43653()