PSCA Antibody (741009)

PSCA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human PSCA Monoclonal Antibody (Catalog # MAB7636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

PSCA Antibody (741009) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PSCA
  Leu21-Ser95
  Accession # O43653
• Specificity: Detects human PSCA in direct ELISAs.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSCA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PSCA Antibody (741009)

• PRO232
• prostate stem cell antigen
• PSCA

Background

PSCA (prostate stem cell antigen) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein of the Thy-1/Ly-6 family. PSCA is expressed in differentiating cells and epithelial cells of the prostate, bladder, kidney, skin, esophagus, stomach and placenta. It may be up- or down-regulated in cancer and may promote or suppress the tumor, depending on location. Mature human PSCA (amino acids 21-95) shares approximately 65% amino acid sequence identity with mouse and rat PSCA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSCA Antibody (MAB7636)(1)

Publication using MAB7636

• Tomoaki Furuta, Tatsuya Oda, Kayo Kiyoi, Ozawa Yusuke, Sota Kimura, Ko Kurimori et al. Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells Cancer Science 2021-09-01 [PMID: 34115906] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: PSCA
• Uniprot:
  • O43653()