PrPC Antibody (SC57-05)

Images

 
Western Blot: PrPC Antibody (SC57-05) [NBP2-67569] - Western blot analysis of PrPC protein on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: PrPC Antibody (SC57-05) [NBP2-67569] - Staining PrPC in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: PrPC Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded rat brain tissue using anti-PrPC antibody. Counter stained with hematoxylin.
Flow Cytometry: PrPC Antibody (SC57-05) [NBP2-67569] - Analysis of SH-SY-5Y cells with PrPC antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Western Blot: PrPC Antibody (SC57-05) [NBP2-67569] - Analysis of PrPC on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: PrPC Antibody (SC57-05) [NBP2-67569] - Staining PrPC in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: PrPC Antibody (SC57-05) [NBP2-67569] - Staining PrPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: PrPC Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded mouse brain tissue using anti-PrPC antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SC57-05
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PrPC Antibody (SC57-05) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human PrPC aa 200-249 / 253. (SwissProt: P04156 Human; SwissProt: P04925 Mouse; SwissProt: P13852 Rat)
Localization
Golgi apparatus, Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRNP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:800
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for PrPC Antibody (SC57-05)

  • CD230
  • CJD
  • fatal familial insomnia)
  • GSS
  • prion protein (p27-30)
  • prion protein PrP
  • prion protein
  • prion-related protein
  • PRIPMGC26679

Background

Prion diseases are transmissible neurodegenerative disorders which affect a range of mammalian species. In humans they can be inherited and sporadic as well as acquired by exposure to human prions. Prions appear to be composed principally of a conformational isomer of host-encoded prion protein and propagate by recruitment of cellular prion protein (1). The function of the cellular prion protein (PrP) is still poorly understood. It has been proposed that one unprecedented role for PrP is against Bax-mediated neuronal apoptosis. It has been shown that PrP potently inhibits Bax-induced cell death in human primary neurons (2). An impaired synaptic inhibition may be involved in the epileptiform activity seen in Creutzfeldt-Jakob and other neurodegenerative diseases and it is believed that loss of function of PrP may contribute to the early synaptic loss and neuronal degeneration seen in these diseases (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PrPC Antibody (NBP2-67569) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PrPC Products

Array NBP2-67569

Bioinformatics Tool for PrPC Antibody (NBP2-67569)

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Diseases for PrPC Antibody (NBP2-67569)

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Bioinformatics

Gene Symbol PRNP