PRP19 Antibody (SC06-53)

Western Blot: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: PRP19 Antibody (SC06-53) [NBP2-67597] - Staining PRP19 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of paraffin-embedded mouse kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.

Flow Cytometry: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of Hela cells with PRP19 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PRP19 Antibody (SC06-53) [NBP2-67597] - Staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PRP19 Antibody (SC06-53) [NBP2-67597] - Staining PRP19 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PRP19 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of paraffin-embedded human kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of paraffin-embedded human lung cancer tissue using anti-PRP19 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of paraffin-embedded mouse brain tissue using anti-PRP19 antibody. Counter stained with hematoxylin.

Product Discontinued

PRP19 Antibody (SC06-53) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: recombinant protein within Human PRP19 aa 380-524 / 504. (SwissProt: Q9UMS4 Human; SwissProt: Q99KP6 Mouse; SwissProt: Q9JMJ4 Rat)
• Localization: Nucleus, Cytoplasm, Lipid droplet.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRPF19
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PRP19 Antibody (SC06-53)

• hPSO4
• NMP200SNEV
• Nuclear matrix protein 200
• nuclear matrix protein NMP200 related to splicing factor PRP19
• pre-mRNA-processing factor 19
• PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
• PRP19PRP19/PSO4 homolog (S. cerevisiae)
• PSO4PRP19/PSO4 homolog
• Senescence evasion factor
• UBOX4

Background

In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF19