Prox1 Antibody (5G10) - BSA Free

Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Prox1 Antibody (5G10) [NBP1-30045] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.

Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - An intracellular stain was performed on HepG2 cells with Prox1 [5G10] Antibody NBP1-30045AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunohistochemistry-Paraffin: Prox1 Antibody (5G10) [NBP1-30045] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in sinusoidal endothelial cells as well as in Kupffer cells.

Immunohistochemistry-Paraffin: Prox1 Antibody (5G10) [NBP1-30045] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot A of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in sinusoidal endothelial cells as well as in Kupffer cells.

Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.

Prox1 (5G10) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rat anti- Prox1 (5G10) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-30045AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: ELISA, Flow, ICC/IF, IHC, CyTOF-ready, IHC
• Clone: 5G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Prox1 Antibody (5G10) - BSA Free Summary

• Immunogen: The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786]
• Epitope: The homeo prospero domain of Prox1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PROX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry Whole-Mount reported in scientific literature (PMID 24654984)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Application Notes: This antibody is CyTOF ready.

Reactivity Notes

It is expected to work with all vertebrates including mammals, birds and fish. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Prox1 Antibody (5G10) - BSA Free

• Homeobox prospero-like protein PROX1
• prospero homeobox 1
• prospero homeobox protein 1
• prospero-related homeobox 1
• Prox1
• PROX-1

Background

Prox1 is a prospero related homeobox gene. Prox1 may play a fundamental role in early development of the CNS. It may also regulate gene expression and development of postmitotic undifferentiated young neurons. Prox1 is expressed in CNS, eye, pancreas, liver and heart tissues, and it is one of the most specific and reliable markers for lymphatic endothelial cells. On a subcellular level Prox1 resides in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prox1 Antibody (NBP1-30045)(6)

Publications using NBP1-30045

• Iskusnykh IY, Fattakhov N, Li Y et al. Lmx1a is a master regulator of the cortical hem Elife 2023-09-19 [PMID: 37725078] (Immunohistochemistry Whole-Mount, Rat)
• Rütsche D, Nanni M, Rüdisser S et Al. Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co-Engineering of Blood and Lymphatic Vasculature Adv Mater 2023-04-21 [PMID: 36724374]
• Wang T, Wang Z, de Fabritus L Et al. 1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and cardiac cell development Developmental cell 2021-11-22 [PMID: 34762852]
• Taira Y, Ikuta Y, Inamori S et al. The formation of multiple pituitary pouches from the oral ectoderm causes ectopic lens development in hedgehog signaling-defective avian embryos Dev. Dyn. 2020-07-07 [PMID: 32633438]
• Baptista AP, Gola A, Huang Y et al. Human organotypic lymphatic vessel model elucidates microenvironment-dependent signaling and barrier function Biomaterials 2019-05-25 [PMID: 31154151] (FLOW, Human)
• Sweat RS, Sloas DC, Murfee WL. VEGF-C Induces Lymphangiogenesis and Angiogenesis in the Rat Mesentery Culture Model. Microcirculation 2014-03-22 [PMID: 24654984] (IHC-WhMt, Rat)

FAQs for Prox1 Antibody (NBP1-30045). (Showing 1 - 1 of 1 FAQs).

1. Does this work for IHC on formalin-fixed tissue, or just frozen tissue? We're most interested in reactivity to mouse tissues.
   • I have checked with the lab and it has only been tested on Paraffin embedded tissue and has yet to be tested on frozen tissue. We will guarantee IHC-P and all the species listed on the datasheet under our 100% guarantee. If you plan to test on frozen tissues and wish to report this data to us we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

Bioinformatics

• Gene Symbol: PROX1
• Entrez:
  • 5629(Human)
  • 19130(Mouse)
  • 305066(Rat)
• Uniprot:
  • Q92786()
  • Q92786()
  • P48437(Mouse)