Protocadherin gamma C3 Antibody (926525) [Unconjugated]

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Western blot shows lysates of H4 human neuroglioma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Protocadherin gamma C3 Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
926525
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Protocadherin gamma C3 Antibody (926525) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin gamma C3
Met1-Tyr693
Accession # Q9UN70
Specificity
Detects human Protocadherin gamma C3 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PCDHGC3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin gamma C3 Antibody (926525) [Unconjugated]

  • Cadherin-Like 2
  • PC43
  • PC-43
  • PCDH2
  • PCDH-GAMMA-C3
  • PCDHGC3
  • protocadherin 2
  • Protocadherin 43
  • Protocadherin gamma C3
  • protocadherin gamma subfamily C, 3
  • protocadherin gamma-C3
  • Protocadherin-2
  • protocadherin-43

Background

Protocadherin  gamma  C3 is a member of the gamma  subgroup of clustered protocadherins (1). Like other gamma  protocadherins, mature Protocadherin  gamma  C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin  gamma  C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA2+ -dependent homophilic interaction (4). MMP-mediated shedding of gamma  protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin  gamma  C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma  gene cluster is embryonic lethal in mice (9). Protocadherin  gamma  C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin  gamma  gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma  isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma  gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma  gene cluster results in dendritic arborization defects (16). The protocadherin gamma  subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).
  1. Yagi, T. (2008) Dev. Growth Differ. 50:S131.
  2. Sano, K. et al. (1993) EMBO J. 12:2249.
  3. Kohmura, N. et al. (1998) Neuron 20:1137.
  4. Obata, S. et al. (1995) J. Cell Sci. 108:3765.
  5. Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.
  6. Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.
  7. Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
  8. Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.
  9. Wang, X. et al. (2002) Neuron 36:843.
  10. Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.
  11. Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.
  12. Lefebvre, J.L. et al. (2008) Development 135:4141.
  13. Prasad, T. et al. (2008) Development 135:4153.
  14. Lin, C. et al. (2010) J. Biol. Chem. 285:41675.
  15. Chen, W.V. et al. (2012) Neuron 75:402.
  16. Garrett, A.M. et al. (2012) Neuron 74:269.
  17. Lobas, M.A. et al. (2012) J. Neurochem. 120:913.
  18. Ledderose, J. et al. (2013) Sci. Rep. 3:1514.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PCDHGC3
Uniprot