Protocadherin-18 Antibody

Images

 
Western Blot: Protocadherin-18 Antibody [NBP1-77005] - Analysis in HepG2 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: Protocadherin-18 Antibody [NBP1-77005] - Immunofluorescence of PCDH18 in Mouse Brain cells with PCDH18 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Protocadherin-18 Antibody [NBP1-77005] - Mouse brain tissue with PCDH18 antibody at 2.5 ug/ml.
Western Blot: Protocadherin-18 Antibody [NBP1-77005] - Raji cell lysate with PCDH18 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Protocadherin-18 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH18. The immunogen is located within amino acids 50 - 100 of PCDH18.
Specificity
At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCDH18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
Protocadherin-18 Peptide (NBP1-77005PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protocadherin-18 Antibody

  • KIAA1562
  • PCDH68L1
  • protocadherin 18
  • Protocadherin18
  • Protocadherin-18

Background

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development. At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PCDH18
Entrez
Uniprot