Protein O-Fucosyltransferase 1/POFUT1 Antibody

Images

 
Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP1-76940] - Human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunohistochemistry-Paraffin: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP1-76940] - Human heart tissue with POFUT1 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Protein O-Fucosyltransferase 1/POFUT1 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1. The immunogen is located within amino acids 90 - 140 of POFUT1.
Specificity
At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POFUT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Protein O-Fucosyltransferase 1/POFUT1 Peptide (NBP1-76940PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein O-Fucosyltransferase 1/POFUT1 Antibody

  • FucT-1
  • FUT12
  • FUT12EC 2.4.1.221
  • GDP-fucose protein O-fucosyltransferase 1
  • KIAA0180MGC2482
  • o-fucosyltransferase protein
  • O-Fuc-T
  • O-FucT-1
  • O-FUT
  • Peptide-O-fucosyltransferase 1
  • POFUT1
  • Protein OFucosyltransferase 1
  • Protein O-Fucosyltransferase 1

Background

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POFUT1
Entrez
Uniprot