Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP1-76940] - Human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry: Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free [NBP1-76940] - Immunohistochemistry of Protein O-Fucosyltransferase 1/POFUT1 in human heart tissue with POFUT antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free (NBP1-76940) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1. The immunogen is located within amino acids 90 - 140 of POFUT1. Amino Acid Squence: DFMEKLAPTHWPPEKR
• Specificity: At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POFUT1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Protein O-Fucosyltransferase 1/POFUT1 Peptide (NBP1-76940PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

• FucT-1
• FUT12
• FUT12EC 2.4.1.221
• GDP-fucose protein O-fucosyltransferase 1
• KIAA0180MGC2482
• o-fucosyltransferase protein
• O-Fuc-T
• O-FucT-1
• O-FUT
• Peptide-O-fucosyltransferase 1
• POFUT1
• Protein OFucosyltransferase 1
• Protein O-Fucosyltransferase 1

Background

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POFUT1
• Entrez:
  • 23509(Human)
  • 140484(Mouse)
• Uniprot:
  • Q9H488(Human)