Protein Kinase D2 Antibody - BSA Free

Immunohistochemistry: Protein Kinase D2 Antibody - BSA Free [NBP3-46893] - Immunohistochemistry of paraffin-embedded human colon cancer using (Protein Kinase D2 antibody) at dilution of 1:100

Western Blot: Protein Kinase D2 Antibody - BSA Free [NBP3-46893] - Human brain tissue were subjected to SDS PAGE followed by western blot with (Protein Kinase D2 antibody) at dilution of 1:500

Immunoprecipitation: Protein Kinase D2 Antibody - BSA Free [NBP3-46893] - IP Result of anti-Protein Kinase D2 (IP:, 4ug; Detection: 1:500) with HeLa cells lysate 1200ug.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Protein Kinase D2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Protein Kinase D2 Antibody - BSA Free (NBP3-46893) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: protein kinase D2. (Uniprot# Q9BZL6)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Protein Kinase D2 Antibody - BSA Free

• DKFZP586E0820
• EC 2.7.11
• EC 2.7.11.13
• nPKC-D2
• PKD2HSPC187
• PRKD2
• protein kinase D2
• serine/threonine-protein kinase D2

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKD2