Protein Kinase D2 Antibody - BSA Free

Western Blot: Protein Kinase D2 Antibody [NBP2-58315] - Analysis using Anti-PRKD2 antibody NBP2-58315 (A) shows similar pattern to independent antibody NBP1-84139 (B).

Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-58315] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Protein Kinase D2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Protein Kinase D2 Antibody - BSA Free (NBP2-58315) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSS
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Protein Kinase D2 Recombinant Protein Antigen (NBP2-58315PEP)

Reactivity Notes

Mouse 88%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Protein Kinase D2 Antibody - BSA Free

• DKFZP586E0820
• EC 2.7.11
• EC 2.7.11.13
• nPKC-D2
• PKD2HSPC187
• PRKD2
• protein kinase D2
• serine/threonine-protein kinase D2

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKD2