Protein Kinase D2 Antibody

Images

 
Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Protein Kinase D2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 320 and 400.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunoprecipitation 2- 10 ug/mg of lysate
Application Notes
This antibody can be used for Western blot and immunoprecipitation. *Suggested Working Dilutions: Western blot: 1:500-1:5,000 Immunoprecipitation: 1-4 ug/mg of lysate Immunohistochemistry: not tested *Optimal working dilutions should be determined experimentally by the investigator.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-636.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein Kinase D2 Antibody

  • DKFZP586E0820
  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-D2
  • PKD2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2

Background

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for Protein Kinase D2 Antibody (NB100-636)(8)


Showing Publications 1 - 8 of 8.
Publications using NB100-636 Applications Species
Zheng H, Qian J, Baker DP et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 2011 Oct [PMID: 21865166]
Azoitei N, Kleger A, Schoo N et al. Protein kinase D2 is a novel regulator of glioblastoma growth and tumor formation. Neuro Oncol 2011 Jul [PMID: 21727210]
Zheng H, Qian J, Varghese B et al. Ligand-stimulated downregulation of the alpha interferon receptor: role of protein kinase D2. Mol Cell Biol 2011 Feb [PMID: 21173164]
Pusapati GV, Krndija D, Armacki M et al. Role of the second cysteine-rich domain and Pro275 in protein kinase D2 interaction with ADP-ribosylation factor 1, trans-Golgi network recruitment, and protein transport. Mol Biol Cell 2010 Mar [PMID: 20089835]
Yuan J, Lugea A, Zheng L et al. Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 2008 Dec [PMID: 18845574]
Sharlow ER, Giridhar KV, LaValle CR et al. Potent and selective disruption of protein kinase D functionality by a benzoxoloazepinolone. J Biol Chem 2008 Nov [PMID: 18829454]
Bossard C, Bresson D, Polishchuk RS et al. Dimeric PKD regulates membrane fission to form transport carriers at the TGN. J Cell Biol 2007 Dec [PMID: 18086912]
Liu D, Evans I, Britton G et al. The zinc-finger transcription factor, early growth response 3, mediates VEGF-induced angiogenesis. Oncogene 2008 May [PMID: 18059339]

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKD2
Entrez
Uniprot