Protein Kinase D2 Antibody


Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified more
Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit more
Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IP
1.0 mg/ml

Independent Antibodies


Biological Strategies


Order Details

Protein Kinase D2 Antibody Summary

The immunogen recognized by this antibody maps to a region between residues 320 and 400.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:5000
  • Immunoprecipitation 2- 10 ug/mg of lysate
Application Notes
This antibody can be used for Western blot and immunoprecipitation. *Suggested Working Dilutions: Western blot: 1:500-1:5,000 Immunoprecipitation: 1-4 ug/mg of lysate Immunohistochemistry: not tested *Optimal working dilutions should be determined experimentally by the investigator.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-636.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Protein Kinase D2 Antibody

  • DKFZP586E0820
  • EC 2.7.11
  • EC
  • nPKC-D2
  • PKD2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2


The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC
Species: Hu, Mu
Applications: ICC/IF, PEP-ELISA
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC
Species: Hu, Mu
Applications: WB, IP, ICC
Species: Hu, Rt, Po, Bv, Gt
Applications: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
Species: Hu
Applications: WB, ELISA
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IP

Publications for Protein Kinase D2 Antibody (NB100-636)(8)

Showing Publications 1 - 8 of 8.
Publications using NB100-636 Applications Species
Zheng H, Qian J, Baker DP et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 2011 Oct [PMID: 21865166]
Azoitei N, Kleger A, Schoo N et al. Protein kinase D2 is a novel regulator of glioblastoma growth and tumor formation. Neuro Oncol 2011 Jul [PMID: 21727210]
Zheng H, Qian J, Varghese B et al. Ligand-stimulated downregulation of the alpha interferon receptor: role of protein kinase D2. Mol Cell Biol 2011 Feb [PMID: 21173164]
Pusapati GV, Krndija D, Armacki M et al. Role of the second cysteine-rich domain and Pro275 in protein kinase D2 interaction with ADP-ribosylation factor 1, trans-Golgi network recruitment, and protein transport. Mol Biol Cell 2010 Mar [PMID: 20089835]
Yuan J, Lugea A, Zheng L et al. Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 2008 Dec [PMID: 18845574]
Sharlow ER, Giridhar KV, LaValle CR et al. Potent and selective disruption of protein kinase D functionality by a benzoxoloazepinolone. J Biol Chem 2008 Nov [PMID: 18829454]
Bossard C, Bresson D, Polishchuk RS et al. Dimeric PKD regulates membrane fission to form transport carriers at the TGN. J Cell Biol 2007 Dec [PMID: 18086912]
Liu D, Evans I, Britton G et al. The zinc-finger transcription factor, early growth response 3, mediates VEGF-induced angiogenesis. Oncogene 2008 May [PMID: 18059339]

Reviews for Protein Kinase D2 Antibody (NB100-636) (0)

There are no reviews for Protein Kinase D2 Antibody (NB100-636). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Protein Kinase D2 Antibody (NB100-636) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Protein Kinase D2 Products

Bioinformatics Tool for Protein Kinase D2 Antibody (NB100-636)

Discover related pathways, diseases and genes to Protein Kinase D2 Antibody (NB100-636). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Protein Kinase D2 Antibody (NB100-636)

Discover more about diseases related to Protein Kinase D2 Antibody (NB100-636).

Pathways for Protein Kinase D2 Antibody (NB100-636)

View related products by pathway.

PTMs for Protein Kinase D2 Antibody (NB100-636)

Learn more about PTMs related to Protein Kinase D2 Antibody (NB100-636).

Blogs on Protein Kinase D2

There are no specific blogs for Protein Kinase D2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Protein Kinase D2 Antibody and receive a gift card or discount.


Gene Symbol PRKD2