Proteasome 20S beta 3 Antibody

Western Blot: Proteasome 20S beta 3 Antibody [NBP2-19953] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE gel, diluted at 1:5000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, RM, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Proteasome 20S beta 3 Antibody Summary

• Description: Novus Biologicals Rabbit Proteasome 20S beta 3 Antibody (NBP2-19953) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human Proteasome 20S beta 3 Recombinant protein.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Mouse (98%), Rat (98%), Porcine (97%), Bovine (99%), Zebrafish (90%), Rhesus Macaque (99%), Canine (99%), Xenopus (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMB3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Proteasome 20S beta 3 Antibody

• EC 3.4.25.1
• HC10-II
• MGC4147
• proteasome (prosome, macropain) subunit, beta type, 3
• Proteasome 20S beta 3
• Proteasome chain 13
• Proteasome component C10-II
• proteasome subunit beta type-3
• Proteasome theta chain
• PSMB3

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PSMB3
• Uniprot:
  • P49720(Human)