Prohibitin 2 Antibody - BSA Free

Western Blot: Prohibitin 2 Antibody [NBP2-50549] - 30 ug mouse Neuro2A whole cell lysate separated by 4-12% PAGE, blotted on PVDF membranes and immunodetected with PHB2 antibody NBP2-50549 at 1:1000 in 5% (w/v) dry milk powder in TBST. WB image submitted by a verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Prohibitin 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Prohibitin 2 Antibody - BSA Free (NBP2-50549) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KLH conjugated synthetic peptide between 106-134 amino acids from human PHB2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Prohibitin 2 Antibody - BSA Free

• BAP
• Bap37
• BCAP37
• B-cell associated protein
• D-Prohibitin
• MGC117268
• p22
• PHB2
• PNAS-141
• Prohibitin 2
• prohibitin-2
• REA
• REAB-cell receptor-associated protein BAP37
• Repressor of estrogen receptor activity

Background

PA5-14132 Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37 kDa, ubiquitous member of the prohibitin family of molecules. PHB2 is found in multiple intracellular compartments. It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER). Human PHB2 is 299 amino acids (aa) in length. It contains a mitochondrial localization signal (aa 1-50), two ER repressor domains (aa 19-49 and aa 150-174), and a central coiled-coil domain (aa 190-238). There may be one splice variant that shows a premature truncation after aa 289. Over aa 89-299, mouse and rat PHB2 are 100% identical to human PHB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Prohibitin 2 Antibody (NBP2-50549) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Marcel Alavi WB Mouse 04/10/2021

Summary

• Application: Western Blot
• Sample Tested: Mouse Neuro2A whole cell lysate
• Species: Mouse
• Lot: WC3235587

Bioinformatics

• Gene Symbol: PHB2