Prohibitin 2 Antibodies
We offer Prohibitin 2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Prohibitin 2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Prohibitin 2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Prohibitin 2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Prohibitin 2 polyclonal antibodies and browse our Prohibitin 2 monoclonal antibody catalog.
Alternate Names for Prohibitin 2 Antibodies
anti-Prohibitin 2 antibody, anti-PHB2 antibody, anti-B-cell associated protein antibody, anti-BAP antibody, anti-Bap37 antibody, anti-BCAP37 antibody, anti-D-prohibitin antibody, anti-MGC117268 antibody, anti-p22 antibody, anti-PNAS-141 antibody, anti-prohibitin-2 antibody, anti-REAB-cell receptor-associated protein BAP37 antibody, anti-Repressor of estrogen receptor activity antibody
14 Results for "prohibitin-2" in Primary Antibodies
HAF016). A specific band was detected for Prohibitin 2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Prohibitin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4794) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prohibitin 2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
![Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-13754-img0012.jpg "Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in human cell line BEWO.")
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody [NBP2-13754] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13754-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0010.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-13754-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0008.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0009.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human tonsil shows strong granular cytoplasmic positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0013.jpg)
![Western Blot: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Western blot analysis of extracts of various cell lines, using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Western-Blot-NBP3-16766-img0004.jpg "Western Blot: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Western blot analysis of extracts of various cell lines, using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunofluorescence analysis of U-2 OS cells using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunocytochemistry-Immunofluorescence-NBP3-16766-img0003.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunohistochemistry-Paraffin-NBP3-16766-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunofluorescence analysis of NIH-3T3 cells using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunocytochemistry-Immunofluorescence-NBP3-16766-img0002.jpg)
![Immunoprecipitation: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunoprecipitation analysis of 300ug extracts of SKOV3 cells using 3ug Prohibitin 2 antibody (NBP3-16766). Western blot was performed from the immunoprecipitate using Prohibitin 2 antibody (NBP3-16766) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunoprecipitation-NBP3-16766-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Confocal imaging of NIH/3T3 cells using Prohibitin 2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16766_rabbit-prohibitin-2-mab-8i10b1-52202511383746.jpg)
![Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Confocal imaging of U-2 OS cells using Prohibitin 2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16766_rabbit-prohibitin-2-mab-8i10b1-5220251242531.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-33463-img0007.jpg "Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody [NBP1-33463] - Analysis of paraformaldehyde-fixed A431, using REA (NBP1-33463) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33463-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Paraffin-embedded mouse urinary bladder . Prohibitin 2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33463-img0008.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-33463-img0006.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33463-img0005.jpg)
![Immunohistochemistry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-298202414262693.jpg "Immunohistochemistry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunocytochemistry analysis of HeLa cells labeling Prohibitin 2 with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-2920241192365.jpg)
![Flow Cytometry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Flow cytometric analysis of HeLa cells labeling Prohibitin 2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32900, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-29202416453249.jpg)
![Western blot analysis on different lysates with NBP3-32900 at 1/1,000 dilution. Lane 1: Huh7 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: PANC-1 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: NIH/3T3 cell lysate (15 µg/Lane) Lane 7: PC-12 cell lysate (15 µg/Lane) Lane 8: Mouse liver tissue lysate (30 µg/Lane) Lane 9: Rat liver tissue lysate (30 µg/Lane) Lane 10: Mouse heart tissue lysate (30 µg/Lane) Lane 11: Rat heart tissue lysate (30 µg/Lane) Lane 12: Mouse testis tissue lysate (30 µg/Lane) Lane 13: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32900 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32900_-western-blot-639204595238478401.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-NB100-1807-img0004.jpg "Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunoprecipitation-NB100-1807-img0001.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP2-50549] - 30 ug mouse Neuro2A whole cell lysate separated by 4-12% PAGE, blotted on PVDF membranes and immunodetected with PHB2 antibody NBP2-50549 at 1:1000 in 5% (w/v) dry milk powder in TBST. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-50549-img0001.jpg "Western Blot: Prohibitin 2 Antibody [NBP2-50549] - 30 ug mouse Neuro2A whole cell lysate separated by 4-12% PAGE, blotted on PVDF membranes and immunodetected with PHB2 antibody NBP2-50549 at 1:1000 in 5% (w/v) dry milk powder in TBST. WB image submitted by a verified customer review.")
![Western Blot: Prohibitin 2 Antibody [NB100-1809] - Detection of Human and Mouse REA on HeLa whole cell lysate using NB100-1809. REA was also immunoprecipitated using rabbit anti-REA antibodies NB100-1807 and NB100-1808.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NB100-1809-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Prohibitin 2 Antibody [NB100-1809] - Detection of Human and Mouse REA on HeLa whole cell lysate using NB100-1809. REA was also immunoprecipitated using rabbit anti-REA antibodies NB100-1807 and NB100-1808.")
![Western Blot: Prohibitin 2 Antibody (SR1516) [NBP3-22147] - Western blot analysis using NBP3-22147 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22147_rabbit-prohibitin-2-mab-sr1516-672023106283.jpg "Western Blot: Prohibitin 2 Antibody (SR1516) [NBP3-22147] - Western blot analysis using NBP3-22147 in HeLa cell lysate.")
![Western Blot: Prohibitin 2 Antibody [NBP1-98312] - WB Suggested Anti-Phb2 Antibody. Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-98312-img0004.jpg "Western Blot: Prohibitin 2 Antibody [NBP1-98312] - WB Suggested Anti-Phb2 Antibody. Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience.")
![Western Blot: Prohibitin 2 Antibody [NBP1-98312] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-98312-img0003.jpg)
![Immunoprecipitation: Prohibitin 2 Antibody [NBP1-98312] - NT2 CELL/BRAIN TISSUE](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunoprecipitation-NBP1-98312-img0002.jpg)
![ELISA: Prohibitin 2 Antibody (2D3) [H00011331-M02] - Detection limit for recombinant GST tagged PHB2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-2D3-ELISA-H00011331-M02-img0002.jpg "ELISA: Prohibitin 2 Antibody (2D3) [H00011331-M02] - Detection limit for recombinant GST tagged PHB2 is 1 ng/ml as a capture antibody.")
