Progesterone R/NR3C3 Antibody

Product Discontinued

Progesterone R/NR3C3 Antibody Summary

• Immunogen: Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).
• Localization: Nuclear
• Specificity: Human progesterone receptor (PR) exists in two forms: 116kDa (B-form) and 81kDa (A-form). It acts as a ligand activated transcription factor to regulate expression of the target genes. Null mutation in PR gene leads to pleiotropic reproductive abnormalities.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: Sodium Azide
• Concentration: 2.92 mg/ml
• Purity: IgG purified

Alternate Names for Progesterone R/NR3C3 Antibody

• NR3C3
• NR3C3PRNuclear receptor subfamily 3 group C member 3
• PGR
• Progesterone R
• progesterone receptor
• ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Progesterone R/NR3C3 Antibody (NB120-15509). (Showing 1 - 1 of 1 FAQ).

1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
   • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Bioinformatics

• Gene Symbol: PGR
• Entrez:
  • 5241(Human)