Progesterone R/NR3C3 Antibody (SPM566) [DyLight 350] Summary
| Immunogen |
Recombinant human Progesterone Receptor protein |
| Localization |
Nuclear |
| Marker |
Marker of Progestin Dependence |
| Specificity |
This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues. |
| Isotype |
IgG1 Kappa |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
PGR |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Western Blot
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
|
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Notes
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Progesterone R/NR3C3 Antibody (SPM566) [DyLight 350]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Progesterone R/NR3C3 Antibody (NBP2-34429UV) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Progesterone R/NR3C3 Antibody (NBP2-34429UV). (Showing 1 - 1 of 1 FAQ).
-
I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
- We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.
Secondary Antibodies
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Isotype Controls
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Other Available Formats
Additional Progesterone R/NR3C3 Products
Bioinformatics Tool for Progesterone R/NR3C3 Antibody (NBP2-34429UV)
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Diseases for Progesterone R/NR3C3 Antibody (NBP2-34429UV)
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Blogs on Progesterone R/NR3C3
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/fullsize2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0004.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: AKR1B1 Antibody [NBP1-53144] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize2/AKR1B1 Antibody-Western Blot-NBP1-53144-img0004.jpg)
![Immunohistochemistry: AKR1B1 Antibody [NBP1-53144] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Immunohistochemistry-NBP1-53144-img0003.jpg)
![Western Blot: AKR1B1 Antibody [NBP1-53144] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Western-Blot-NBP1-53144-img0002.jpg)
![Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.](http://images.novusbio.com/fullsize2/GR-NR3C1-Antibody-(BuGR2)-Western-Blot-NB300-731-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/GR-NR3C1-Antibody-(BuGR2)-Immunocytochemistry-Immunofluorescence-NB300-731-img0007.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/GR-NR3C1-Antibody-(BuGR2)-Flow-Cytometry-NB300-731-img0018.jpg)
![Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize2/MID1-Antibody-Western-Blot-NBP1-26612-img0002.jpg)
![Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Western-Blot-NBP2-38530-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38530-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human ovary.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-38530-img0012.jpg)
![Western Blot: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:5000.](http://images.novusbio.com/fullsize2/ER-beta-NR3A2-Antibody-(14C8)-Western-Blot-NB200-305-img0006.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Human breast carcinoma. Estrogen Receptor beta stained by Estrogen Receptor beta antibody [14C8] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize2/ER-beta-NR3A2-Antibody-(14C8)-Immunohistochemistry-Paraffin-NB200-305-img0007.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor beta Antibody (14C8) [NB200-305] - Infiltrating lobular carcinoma of the breast.](http://images.novusbio.com/fullsize2/Estrogen Receptor beta Antibody (14C8)-Immunohistochemistry-Paraffin-NB200-305-img0003.jpg)
![Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/DBT-Antibody-Western-Blot-NBP1-89522-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89522-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon.](http://images.novusbio.com/fullsize2/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0015.jpg)
![Immunohistochemistry-Paraffin: NEURL Antibody [NBP1-92177] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize2/NEURL-Antibody-Immunohistochemistry-Paraffin-NBP1-92177-img0003.jpg)
![Western Blot: SLC17A5 Antibody [NBP2-20383] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/SLC17A5 Antibody-Western Blot-NBP2-20383-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SLC17A5 Antibody [NBP2-20383] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: SLC17A5 protein stained by SLC17A5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize2/SLC17A5 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20383-img0003.jpg)
![Immunohistochemistry-Paraffin: Sialin/SLC17A5 Antibody [NBP2-20383] - Mouse lung diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize2/Sialin-SLC17A5-Antibody-Immunohistochemistry-Paraffin-NBP2-20383-img0004.jpg)
![Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1 Antibody (1B4)-Western Blot-NBP2-02623-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.](http://images.novusbio.com/fullsize/RanGAP1 Antibody (1B4)-Immunocytochemistry-Immunofluorescence-NBP2-02623-img0008.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RanGAP1 Antibody (1B4)-Immunohistochemistry-Paraffin-NBP2-02623-img0007.jpg)
![Western Blot: TFF1/pS2 Antibody [NBP1-90813] - Analysis in ysate from HEK293 cells. Image from a verified customer review.](http://images.novusbio.com/fullsize2/TFF1-pS2-Antibody-Western-Blot-NBP1-90813-img0021.jpg)
![Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP1-90813] - Staining in human stomach and liver tissues using anti-TFF1 antibody. Corresponding TFF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/TFF1-pS2-Antibody-Immunohistochemistry-Paraffin-NBP1-90813-img0017.jpg)
![Western Blot: TFF1/pS2 Antibody [NBP1-90813] - Analysis in control (vector only transfected HEK293T lysate) and TFF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/TFF1-pS2 Antibody-Western Blot-NBP1-90813-img0020.jpg)
