| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IHC-Fr, IHC-P |
| Clone | SPM566 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 350 |
| Immunogen | Recombinant human Progesterone Receptor protein |
| Localization | Nuclear |
| Marker | Marker of Progestin Dependence |
| Specificity | This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PGR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![Progesterone R/NR3C3 Antibody (SPM566) [DyLight 350]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Progesterone R/NR3C3 Antibody (SPM566) [DyLight 350]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-34429AF350 | |
| Alexa Fluor 405 | NBP2-34429AF405 | |
| Alexa Fluor 488 | NBP2-34429AF488 | |
| Alexa Fluor 532 | NBP2-34429AF532 | |
| Alexa Fluor 594 | NBP2-34429AF594 | |
| Alexa Fluor 647 | NBP2-34429AF647 | |
| Alexa Fluor 700 | NBP2-34429AF700 | |
| Alexa Fluor 750 | NBP2-34429AF750 | |
| Biotin | NBP2-34429B | |
| DyLight 350 | NBP2-34429UV | |
| DyLight 405 | NBP2-34429V | |
| DyLight 488 | NBP2-34429G | |
| DyLight 550 | NBP2-34429R | |
| DyLight 594 | NBP2-34429DL594 | |
| DyLight 650 | NBP2-34429C | |
| DyLight 680 | NBP2-34429FR | |
| DyLight 755 | NBP2-34429IR | |
| FITC | NBP2-34429F | |
| HRP | NBP2-34429H | |
| Janelia Fluor 549 | NBP2-34429JF549 | |
| Janelia Fluor 646 | NBP2-34429JF646 |
Diseases for Progesterone R/NR3C3 Antibody (NBP2-34429UV)Discover more about diseases related to Progesterone R/NR3C3 Antibody (NBP2-34429UV).
| Pathways for Progesterone R/NR3C3 Antibody (NBP2-34429UV)View related products by pathway.
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| Gene Symbol | PGR |
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/images2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89146-img0006.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0016.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0010.jpg)
![Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.](http://images.novusbio.com/images2/GR-NR3C1-Antibody-(BuGR2)-Western-Blot-NB300-731-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/GR-NR3C1-Antibody-(BuGR2)-Immunocytochemistry-Immunofluorescence-NB300-731-img0007.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/GR-NR3C1-Antibody-(BuGR2)-Flow-Cytometry-NB300-731-img0018.jpg)
![Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/images2/MID1-Antibody-Western-Blot-NBP3-05061-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/MID1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05061-img0001.jpg)
![Western Blot: MID1 Antibody [NBP3-05061] - Analysis of extracts of various cell lines, using MID1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/images2/MID1 Antibody-Western Blot-NBP3-05061-img0002.jpg)
![Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Western-Blot-NBP2-38530-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38530-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining in human adrenal gland and lymph node tissues using NBP2-38530 antibody. Corresponding FDXR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-38530-img0019.jpg)
![Western Blot: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:5000.](http://images.novusbio.com/images2/ER-beta-NR3A2-Antibody-(14C8)-Western-Blot-NB200-305-img0006.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Human breast carcinoma. Estrogen Receptor beta stained by Estrogen Receptor beta antibody [14C8] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/images2/ER-beta-NR3A2-Antibody-(14C8)-Immunohistochemistry-Paraffin-NB200-305-img0007.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor beta Antibody (14C8) [NB200-305] - Infiltrating lobular carcinoma of the breast.](http://images.novusbio.com/images2/Estrogen Receptor beta Antibody (14C8)-Immunohistochemistry-Paraffin-NB200-305-img0003.jpg)
![Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/DBT-Antibody-Western-Blot-NBP1-89522-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89522-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon.](http://images.novusbio.com/images2/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0015.jpg)
![Western Blot: NEURL Antibody [NBP2-99299] - Anti-NEURL rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa](http://images.novusbio.com/images2/NEURL-Antibody-Western-Blot-NBP2-99299-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NEURL Antibody [NBP2-99299] - Immunofluorescence staining of NEURL in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NEURL polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/images2/NEURL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99299-img0001.jpg)
![Western Blot: SLC17A5 Antibody [NBP2-20383] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/SLC17A5 Antibody-Western Blot-NBP2-20383-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SLC17A5 Antibody [NBP2-20383] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: SLC17A5 protein stained by SLC17A5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/images2/SLC17A5 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20383-img0003.jpg)
![Immunohistochemistry-Paraffin: Sialin/SLC17A5 Antibody [NBP2-20383] - Mouse lung diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/images2/Sialin-SLC17A5-Antibody-Immunohistochemistry-Paraffin-NBP2-20383-img0004.jpg)
![Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.](http://images.novusbio.com/images/RanGAP1 Antibody (1B4)-Western Blot-NBP2-02623-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.](http://images.novusbio.com/images/RanGAP1 Antibody (1B4)-Immunocytochemistry-Immunofluorescence-NBP2-02623-img0008.jpg)
![Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.](http://images.novusbio.com/images/RanGAP1 Antibody (1B4)-Immunohistochemistry-Paraffin-NBP2-02623-img0007.jpg)
![Western Blot: TFF1/pS2 Antibody [NBP1-90813] - Analysis in ysate from HEK293 cells. Image from a verified customer review.](http://images.novusbio.com/images2/TFF1-pS2-Antibody-Western-Blot-NBP1-90813-img0021.jpg)
![Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP1-90813] - Staining in human stomach and liver tissues using anti-TFF1 antibody. Corresponding TFF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/TFF1-pS2-Antibody-Immunohistochemistry-Paraffin-NBP1-90813-img0017.jpg)
![Western Blot: TFF1/pS2 Antibody [NBP1-90813] - Analysis in control (vector only transfected HEK293T lysate) and TFF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/TFF1-pS2 Antibody-Western Blot-NBP1-90813-img0020.jpg)