Progesterone R/NR3C3 Antibody (SP42)

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Endometrium

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Breast

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Cervix

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Ovarian Adenocarcinoma

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Uterus

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Prostate

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Human Pancreas

Immunohistochemistry-Paraffin: Rabbit Monoclonal Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Progesterone receptor immunoreactivity in an FFPE section of mouse vagina. NBP1-49806 was diluted to 0.25ug per mL and was left on tissue sections for 30m at room temperature. No antigen retrieval was needed. Image from a verified customer review.

Immunohistochemistry-Paraffin: Rabbit Monoclonal Progesterone R/NR3C3 Antibody (SP42) [NBP1-49806] - Progesterone receptor immunoreactivity in an FFPE section of equine uterus. NBP1-49806 was diluted to 0.25ug per mL and was left on tissue sections for 30m at room temperature. No antigen retrieval was necessary. Image from a verified customer review.

Product Discontinued

Progesterone R/NR3C3 Antibody (SP42) Summary

• Description: For a positive control use Breast carcinoma.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein encoding human progesterone receptor 412-526 aa.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PGR
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Porcine, Bovine, Rabbit. Mouse and equine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS/1% BSA buffer pH 7.6.
• Preservative: Sodium Azide
• Purity: Protein A or G purified

Notes

For a positive control use Breast carcinoma.

Alternate Names for Progesterone R/NR3C3 Antibody (SP42)

• NR3C3
• NR3C3PRNuclear receptor subfamily 3 group C member 3
• PGR
• Progesterone R
• progesterone receptor
• ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Progesterone R/NR3C3 Antibody (NBP1-49806) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Horse.

reviewed by: Verified Customer IHC-P Mouse 12/04/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Vagina
• Species: Mouse
• Lot: 150423LVA

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***No antigen retrieval was needed for some sections. Brief 5m heat induced epitope retrieval with a citrate or EDTA buffer increased intensity of the label but also produced cytoplasmic labeling which may be nonspecific. No signal was observed in tissue sections that underwent proteinase induced epitope retrieval with 5m proteinase K at room temperature.

reviewed by: Verified Customer IHC-P Horse 12/04/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Uterus
• Species: Horse
• Lot: 150423LVA

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** No antigen retrieval was needed for some sections. Brief 5m heat induced epitope retrieval increased the intensity of the label but also produced cytoplasmic labeling which may be nonspecific. No signal was observed in tissue sections that underwent proteinase induced epitope retrieval with 5m proteinase K.

FAQs for Progesterone R/NR3C3 Antibody (NBP1-49806). (Showing 1 - 1 of 1 FAQ).

1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
   • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Bioinformatics

• Gene Symbol: PGR