Progesterone R/NR3C3 Antibody (alpha PR-22) - BSA Free

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Western Blot: Progesterone R/NR3C3 Antibody (alpha PR-22) [NB120-2767] - WB analysis using this antibody.
Immunofluorescence: Progesterone R/NR3C3 Antibody (alpha PR-22) [NB120-2767] - IF analysis of Progesterone Receptor (green) in T47D cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-2767
    • Availability
      Product Discontinued

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Progesterone R/NR3C3 Antibody (alpha PR-22) - BSA Free Summary

Immunogen
Progesterone receptor purified from chick oviduct cytosol.
Localization
Nuclear
Specificity
Detects the A and B forms of progesterone receptor (PR) from chicken and some species of turtles. This does not cross-react with estrogen receptor or glucocorticoid receptor.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PGR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunofluorescence 10 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 78 kDa protein representing the A form and a 110 kDa protein representing the B form of PR. The proportion of receptor bound by this product is significantly greater than NB120-2765 due to the binding of both the A and B form of PR. Protein A can effectively be used with this product in IP experiments.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute to desired concentration.

Alternate Names for Progesterone R/NR3C3 Antibody (alpha PR-22) - BSA Free

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Progesterone R/NR3C3 Antibody (NB120-2767). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Secondary Antibodies

 

Isotype Controls

Additional Progesterone R/NR3C3 Products

Bioinformatics Tool for Progesterone R/NR3C3 Antibody (NB120-2767)

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Diseases for Progesterone R/NR3C3 Antibody (NB120-2767)

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PTMs for Progesterone R/NR3C3 Antibody (NB120-2767)

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Bioinformatics

Gene Symbol PGR