Recombinant Human Profilin 1 Protein

SDS-Page: Recombinant Human Profilin 1 Protein [NBP1-30215] - Profilin 1, 15.0 kDa (140aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Profilin 1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-140 of Human Profilin 1
  
  

  Source: E.coli

  Amino Acid Sequence: MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PFN1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Profilin 1 Protein

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant Profilin1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Profilin 1 Recombinant Protein (NBP1-30215)(1)

Publication using NBP1-30215

• Ahn SB, Kamath KS, Mohamedali A et al. Use of a Recombinant Biomarker Protein DDA Library Increases DIA Coverage of Low Abundance Plasma Proteins Journal of proteome research 2021-03-22 [PMID: 33752330]

Bioinformatics

• Gene Symbol: PFN1