Profilin 1 Antibody

Product Discontinued

Profilin 1 Antibody Summary

• Immunogen: Porcine and human platelet Profilin 1
• Specificity: NB200-162 is specific for profilin I protein but also reacts with profilin II.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1-0.2 ug/ml
• Application Notes: A band should be seen at ~12-15 kDa, in Western blotting. Use tricine gels (13% acrylamide) or 15% Laemmli gels. **The given dilutions refer to the analysis of mammalian cells and tissues with intermediate levels of profilin expression.

Reactivity Notes

human, porcine, mouse, rat, and marsupial profilin

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody is also supplied with Human platelet protein (500 ug at 5mg/ml) in SDS sample buffer. Use 5 ul (25 ug) per lane for Western blotting of tricine gels (13% acrylamide)38 or 15% Laemmli gels.

Alternate Names for Profilin 1 Antibody

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin 1 is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Profilin 1 Antibody (NB200-162)(6)

Publications using NB200-162

• Scotto di Carlo F, Russo S, Muyas F et al. Profilin 1 deficiency drives mitotic defects and impairs genome stability bioRxiv 2022-02-01 [PMID: 36599901] (ICC/IF, WB, Mouse, Human)
• Stoeckelhuber M, Scherer EQ, Janssen KP, Slotta-Huspenina J, Loeffelbein DJ, Rohleder NH, Nieberler M, Hasler R, Kesting MR. The human submandibular gland: immunohistochemical analysis of SNAREs and cytoskeletal proteins. J Histochem Cytochem;60(2):110-20. 2012-02-01 [PMID: 22131313]
• Evans, N J, Walker, J W. (2006) Endothelin-1 Mobilizes Profilin-1-Bound PIP2 in Cardiac Muscle, 231, 882-887. [PMID: 16741017]
• Giesemann et al. A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems. J Biol Chem;274(53):37908-14. 1999-12-31 [PMID: 10608857]
• Hauser et al. Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice. Proc Natl Acad Sci U S A 96(14):8120-5. 1999-07-06 [PMID: 10393958]
• Salazar R, Bell SE, Davis GE. Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro. Exp Cell Res 249(1):22-32. 1999-05-25 [PMID: 10328950]

Bioinformatics

• Gene Symbol: PFN1
• Entrez:
  • 5216(Human)
  • 18643(Mouse)
  • 64303(Rat)