ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein

ProDots® Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 0.1-0.4 μg/mL.

1 μg/lane of ProDots® Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 22 kDa.

Product Discontinued

ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein Summary

• Additional Information: Supplied as ready-to-use 5 ug protein balls
• Details of Functionality: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED₅₀ for this effect is typically 0.1-0.4 μg/mL.
• Source: E. coli-derived human Sonic Hedgehog/Shh protein
  Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met
• Accession #: NP_000184
• N-terminal Sequence: Met
• Protein/Peptide Type: ProDots Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles.
  • 6 months from date of receipt at room temperatureas supplied.
  • 12 months from date of receipt at 2-8 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -80 °C under sterile conditions afterreconstitution.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: For a stock solution, reconstitute at 100 µg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use.

Notes

726244.0

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

Bioinformatics

• Uniprot:
  • NP_000184()